Reviewed, UniProtKB/Swiss-Prot P41833 (IME4_YEAST)

Names and origin

Protein names

Recommended name:
    N6-adenosine-methyltransferase IME4
    EC=2.1.1.62

Gene names

Name:	IME4
Synonyms:	SPO8
Ordered Locus Names:	YGL192W
ORF Names:	G1337

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	N6-methyltransferase that methylates adenosine residues of some mRNAs and is essential to activate sporulation. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, is probably required to initiate sporulation. Positive regulator for IME2.
Catalytic activity	S-adenosyl-L-methionine + m(7)G(5')pppAm = S-adenosyl-L-homocysteine + m(7)G(5')pppm(6)Am.
Subcellular location	NucleusProbable.
Induction	In response to starvation conditions.
Sequence similarities	Belongs to the MT-A70-like family.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mRNA modification Ref.5 Inferred from mutant phenotype. Source: SGD meiosis Ref.1 Inferred from mutant phenotype. Source: SGD
Molecular function	mRNA methyltransferase activity Ref.5 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KAR4	P25583	1	EBI-9210,EBI-9509

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 600	600	N6-adenosine-methyltransferase IME4
Experimental info
	Mutagenesis	348	1	D → A: Impairs ability to sporulate
	Mutagenesis	351	1	W → A: Impairs ability to sporulate

Sequences

	Sequence		Length	Mass (Da)
	P41833-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A99BE7EBA76790BA	FASTA	600	69,396
	10 20 30 40 50 60 MINDKLVHFL IQNYDDILRA PLSGQLKDVY SLYISGGYDD EMQKLRNDKD EVLQFEQFWN 70 80 90 100 110 120 DLQDIIFATP QSIQFDQNLL VADRPEKIVY LDVFSLKILY NKFHAFYYTL KSSSSSCEEK 130 140 150 160 170 180 VSSLTTKPEA DSEKDQLLGR LLGVLNWDVN VSNQGLPREQ LSNRLQNLLR EKPSSFQLAK 190 200 210 220 230 240 ERAKYTTEVI EYIPICSDYS HASLLSTSVY IVNNKIVSLQ WSKISACQEN HPGLIECIQS 250 260 270 280 290 300 KIHFIPNIKP QTDISLGDCS YLDTCHKLNT CRYIHYLQYI PSCLQERADR ETASENKRIR 310 320 330 340 350 360 SNVSIPFYTL GNCSAHCIKK ALPAQWIRCD VRKFDFRVLG KFSVVIADPA WNIHMNLPYG 370 380 390 400 410 420 TCNDIELLGL PLHELQDEGI IFLWVTGRAI ELGKESLNNW GYNVINEVSW IKTNQLGRTI 430 440 450 460 470 480 VTGRTGHWLN HSKEHLLVGL KGNPKWINKH IDVDLIVSMT RETSRKPDEL YGIAERLAGT 490 500 510 520 530 540 HARKLEIFGR DHNTRPGWFT IGNQLTGNCI YEMDVERKYQ EFMKSKTGTS HTGTKKIDKK 550 560 570 580 590 600 QPSKLQQQHQ QQYWNNMDMG SGKYYAEAKQ NPMNQKHTPF ESKQQQKQQF QTLNNLYFAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IME4, a gene that mediates MAT and nutritional control of meiosis in Saccharomyces cerevisiae." Shah J.C., Clancy M.J. Mol. Cell. Biol. 12:1078-1086(1992) [PubMed: 1545790] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GKY5.
[2]	Sakurai M. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene." Clancy M.J., Shambaugh M.E., Timpte C.S., Bokar J.A. Nucleic Acids Res. 30:4509-4518(2002) [PubMed: 12384598] [Abstract] Cited for: FUNCTION, INDUCTION, MUTAGENESIS OF ASP-348 AND TRP-351.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30859 Genomic DNA. Translation: AAA74443.1. D23721 Genomic DNA. Translation: BAA04938.1. X91837 Genomic DNA. Translation: CAA62952.1. Z72714 Genomic DNA. Translation: CAA96904.1.
PIR	S48509.
RefSeq	NP_011323.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1382N.
IntAct	P41833.
Genome annotation databases
Ensembl	YGL192W. Saccharomyces cerevisiae. [Contig view]
GeneID	852683.
GenomeReviews	Gene locus YGL192W in contig Y13135_GR.
KEGG	sce:YGL192W.
NMPDR	fig|4932.3.peg.2426.
Organism-specific databases
CYGD	YGL192w.
SGD	S000003160. IME4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P41833.
Gene expression databases
ArrayExpress	P41833.
GermOnline	YGL192W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007757. MT-A70. [Graphical view]
Pfam	PF05063. MT-A70. 1 hit. [Graphical view]
PROSITE	PS51143. MT_A70. 1 hit. [Graphical view]
ProDom	P41833. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P41833.
ProtoNet	Search...

Entry information

Entry name

IME4_YEAST

Accession

Primary (citable) accession number: P41833

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)