Reviewed, UniProtKB/Swiss-Prot P41984 (ACM3_BOVIN)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M3

Gene names

Name:

CHRM3

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane proteinBy similarity. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane proteinBy similarity. Basolateral cell membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Cellular component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 590	590	Muscarinic acetylcholine receptor M3
Regions
	Topological domain	1 – 67	67	Extracellular Potential
	Transmembrane	68 – 91	24	1 Potential
	Topological domain	92 – 104	13	Cytoplasmic Potential
	Transmembrane	105 – 125	21	2 Potential
	Topological domain	126 – 142	17	Extracellular Potential
	Transmembrane	143 – 164	22	3 Potential
	Topological domain	165 – 184	20	Cytoplasmic Potential
	Transmembrane	185 – 207	23	4 Potential
	Topological domain	208 – 229	22	Extracellular Potential
	Transmembrane	230 – 252	23	5 Potential
	Topological domain	253 – 492	240	Cytoplasmic Potential
	Transmembrane	493 – 513	21	6 Potential
	Topological domain	514 – 527	14	Extracellular Potential
	Transmembrane	528 – 547	20	7 Potential
	Topological domain	548 – 590	43	Cytoplasmic Potential
	Motif	275 – 281	7	Basolateral sorting signal By similarity
Amino acid modifications
	Glycosylation	6	1	N-linked (GlcNAc...) Potential
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	53	1	N-linked (GlcNAc...) Potential
	Disulfide bond	141 ↔ 221		By similarity
Experimental info
	Sequence conflict	424	1	F → S in AAA30653. Ref.2
	Sequence conflict	438	1	A → G Ref.2
	Sequence conflict	440	1	A → G Ref.2
	Sequence conflict	452	1	A → G in AAA30653. Ref.2
	Sequence conflict	461	1	A → G in AAA30653. Ref.2
	Sequence conflict	467	1	F → L in AAA30653. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41984-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4DE04EDE33CCA8D6	FASTA	590	66,103
	10 20 30 40 50 60 MTLHNNNTTS PLFPNISSSW IHGPSDTGLP PGTVTHFGSY NISRAAGNLS SPNGTTSDPL 70 80 90 100 110 120 GGHTIWQVVF IAFLTGVLAL VTIIGNILVI VAFKVNKQLK TVNNYFLLSL ACADLIIGVI 130 140 150 160 170 180 SMNLFTTYII MNRWALGNLA CDLWLSIDYV ASNASVMNLL VISFDRYFSI TRPLTYRAKR 190 200 210 220 230 240 TTKRAGVMIG LAWVISFILW APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF 250 260 270 280 290 300 YMPVTIMTIL YWRIYKETEK RTKELAGLQA SGTEAEAENF VHPTGSSRSC SSYELQQQSM 310 320 330 340 350 360 KRSARRKYGR CHFWFTTKSW KPSAEQMDQD HSSSDSWNNN DAAASLENSA SSDEEDIGSE 370 380 390 400 410 420 TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGSVG LERKPSKLQT QQSMDDGGSF 430 440 450 460 470 480 QKSFSKLPIQ LESAVDTAKA SDVNSSVGKT TATLPLSFKE ATLAKRFALK TRSQITKRKR 490 500 510 520 530 540 MSLIKEKKAA QTLSAILLAF IITWTPYNIM VLVNTFCDSC IPKTYWNLGY WLCYINSTVN 550 560 570 580 590 PVCYALCNKT FRNTFKMLLL CQCDKRKRRK QQYQQRQSVI FHKRVPEQAL

References

[1]	"Cloning and expression of a cDNA encoding bovine muscarinic acetylcholine m3 receptor." Lee P.H., Hodges P.K., Glickman F., Chang K.J. Biochim. Biophys. Acta 1223:151-154(1994) [PubMed: 8061048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Presence of multiple muscarinic receptor subtypes in bovine chromaffin cells - analysis by polymerase chain reaction." Sui A.-L., Chou W.-Y., Kao L.-S. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 327-467. Tissue: Adrenal gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08286 mRNA. Translation: AAA51866.1. L27103 mRNA. Translation: AAA30653.1.
PIR	S47572.
RefSeq	NP_776695.1.
UniGene	Bt.501
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSBTAG00000008059. Bos taurus. [Contig view]
GeneID	281685.
KEGG	bta:281685.
Phylogenomic databases
HOVERGEN	P41984.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001183. Musac_M3_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF37. Musac_M3_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00540. MUSCRINICM3R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P41984. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM3_BOVIN

Accession

Primary (citable) accession number: P41984

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)