Reviewed, UniProtKB/Swiss-Prot P41996 (CPG2_CAEEL)

Names and origin

Protein names

Recommended name:
    Chondroitin proteoglycan-2
Alternative name(s):
    Cytokinesis protein B0280.5

Gene names

Name:	cpg-2
ORF Names:	B0280.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for polar body extrusion during cytokinesis in embryo development. Affects cortical granule size. Has roles in meiotic chromosome segregation, osmotic barrier function and polarization in conjunction with cpg-2. Binds chitin.
Tissue specificity	Expressed in the germline.
Developmental stage	Expressed throughout development but appears to be up-regulated in adults.
Miscellaneous	Worms lacking cpg-2 and cpg-1 exhibit defects in cytokinesis during embryo development more specifically meiotic chromosome segregation, polar-body extrusion, osmotic barrier function and polarization. Embryos lacking cpg-2 and cpg-1 proteins have multiple nuclei lacking plasma membranes and may also have weak egg shells. Oocytes lacking cpg-2 and cpg-1 show cortical granules that are reduced in size.
Sequence similarities	Contains 6 chitin-binding type-2 domains.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Repeat Signal
Ligand	Chitin-binding
Molecular function	Developmental protein
PTM	Glycoprotein Proteoglycan
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chitin metabolic process Inferred from electronic annotation. Source: InterPro cytokinesis after mitosis Ref.4 Inferred from mutant phenotype. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	chitin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 524	506	Chondroitin proteoglycan-2		PRO_0000023617
Regions
	Domain	21 – 78	58	Chitin-binding type-2 1
	Domain	135 – 192	58	Chitin-binding type-2 2
	Domain	244 – 301	58	Chitin-binding type-2 3
	Domain	306 – 361	56	Chitin-binding type-2 4
	Domain	400 – 456	57	Chitin-binding type-2 5
	Domain	469 – 524	56	Chitin-binding type-2 6
	Compositional bias	75 – 258	184	Gly-rich
Amino acid modifications
	Glycosylation	103	1	O-linked (Xyl...) (chondroitin sulfate)
	Glycosylation	119	1	O-linked (Xyl...) (chondroitin sulfate)
	Glycosylation	208	1	O-linked (Xyl...) (chondroitin sulfate)
	Glycosylation	212	1	O-linked (Xyl...) (chondroitin sulfate)
	Glycosylation	355	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Disulfide bond	54 ↔ 67		By similarity
	Disulfide bond	168 ↔ 181		By similarity
	Disulfide bond	277 ↔ 290		By similarity
	Disulfide bond	337 ↔ 350		By similarity
	Disulfide bond	432 ↔ 445		By similarity
	Disulfide bond	500 ↔ 514		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41996-1 [UniParc]. Last modified August 15, 2003. Version 3. Checksum: FFCB9A750385FB34	FASTA	524	53,652
	10 20 30 40 50 60 MKTVAALTLL AFATAANGQF LQDCTNALDG LYALGECEPQ FLTCSGGIAR IMDCPADLIY 70 80 90 100 110 120 NEPLLICDWR HNVIGCEGSG ESSGETSGEG SGESSGEASG EGSGEASGEG SGEASGEGSG 130 140 150 160 170 180 EASGEGSGSG EETVENVCEN LEDGAYSSGG CTTYYFFCTT NTARFLSCPT PLFYDADSQK 190 200 210 220 230 240 CIWKSLVEEC KEDLTITDGS GETSGEGSGE ASGEASGEGS GEASGESSGQ GSGEASGEGS 250 260 270 280 290 300 GELEPTCEGK ADGIHPNGVC STNFLTCSGG IARIMDCPAS LVFNPTILVC DWPRDVAECA 310 320 330 340 350 360 GLPTPQPTCE EDGYFSFGQC SSSFTACTNG RAIVMFCPAG LKFSESTVRC DYESNVSECQ 370 380 390 400 410 420 ETSGEESGEA SGEQSGEGSG EASGEASGES SGEGSGVEEQ NQCVGLDNGL HAIGCSPRVL 430 440 450 460 470 480 SCQNGHVDIF ECPSSLVFND QSLICDYPQT SLKCLIEDTI LIDETPIAAF DCSTDGLFSD 490 500 510 520 GLCSATYHQC TAGQLINFTC AASNAVFSAA NTECVDSSTL LQCH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of novel chondroitin proteoglycans in Caenorhabditis elegans: embryonic cell division depends on CPG-1 and CPG-2." Olson S.K., Bishop J.R., Yates J.R., Oegema K., Esko J.D. J. Cell Biol. 173:985-994(2006) [PubMed: 16785326] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, GLYCOSYLATION AT SER-103; SER-119; SER-208 AND SER-212, FUNCTION.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"A global profile of germline gene expression in C. elegans." Reinke V., Smith H.E., Nance J., Wang J., Van Doren C., Begley R., Jones S.J.M., Davis E.B., Scherer S., Ward S., Kim S.K. Mol. Cell 6:605-616(2000) [PubMed: 11030340] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development." Lee M.-H., Schedl T. Genes Dev. 15:2408-2420(2001) [PubMed: 11562350] [Abstract] Cited for: FUNCTION.
[5]	"The eggshell is required for meiotic fidelity, polar-body extrusion and polarization of the C. elegans embryo." Johnston W.L., Krizus A., Dennis J.W. BMC Biol. 4:35-35(2006) [PubMed: 17042944] [Abstract] Cited for: FUNCTION.
[6]	"Cortical granule exocytosis in C. elegans is regulated by cell cycle components including separase." Bembenek J.N., Richie C.T., Squirrell J.M., Campbell J.M., Eliceiri K.W., Poteryaev D., Spang A., Golden A., White J.G. Development 134:3837-3848(2007) [PubMed: 17913784] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ340624 mRNA. Translation: ABC65812.1. U10438 Genomic DNA. Translation: AAA19083.3.
PIR	T15299.
RefSeq	NP_498551.3.
UniGene	Cel.17405
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P41996.
Genome annotation databases
Ensembl	B0280.5. Caenorhabditis elegans. [Contig view]
GeneID	175991.
KEGG	cel:B0280.5.
Organism-specific databases
WormBase	WBGene00015102. cpg-2.
WormPep	B0280.5. CE31868. [WorfDB]
Gene expression databases
ArrayExpress	P41996.
Family and domain databases
InterPro	IPR002557. Chitin-bd_peritrophin-A. [Graphical view]
Pfam	PF01607. CBM_14. 6 hits. [Graphical view]
SMART	SM00494. ChtBD2. 6 hits. [Graphical view]
PROSITE	PS50940. CHIT_BIND_II. 6 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	890638.

Entry information

Entry name

CPG2_CAEEL

Accession

Primary (citable) accession number: P41996
Secondary accession number(s): Q1A3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 15, 2003
Last modified:	November 25, 2008
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project