Reviewed, UniProtKB/Swiss-Prot P42065 (APPF_BACSU)

Names and origin

Protein names

Recommended name:
    Oligopeptide transport ATP-binding protein appF

Gene names

Name:	appF
Ordered Locus Names:	BSU11370

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transporting tripeptides. Probably responsible for energy coupling to the transport system.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the ABC transporter family. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Competence Peptide transport Protein transport Sporulation Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW oligopeptide transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity Inferred from electronic annotation. Source: InterPro oligopeptide transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Oligopeptide transport ATP-binding protein appF		PRO_0000091932
Regions
	Domain	10 – 261	252	ABC transporter
	Nucleotide binding	53 – 60	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P42065-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2D2D5832B0155946	FASTA	329	37,112
	10 20 30 40 50 60 MTAANQETIL ELRDVKKYFP IRSGLFQRKV GDVKAVDGVS FSLKKGETLG IVGESGCGKS 70 80 90 100 110 120 TAGRTMIRLY KPTEGQILFK GQDISNLSEE KLRKSVRKNI QMVFQDPFAS LNPRKTLRSI 130 140 150 160 170 180 IKEPFNTHNM YTMRERNEKV EELLARVGLH PSFAGRYPHE FSGGQRQRIG IARALTLNPE 190 200 210 220 230 240 LIIADEPVSA LDVSIQPQVI NLMEELQEEF NLTYLFISHD LSVVRHISDR VGVMYLGKMM 250 260 270 280 290 300 ELTGKHELYD NPLHPYTQAL LSSVPVTRKR GSVKRERIVL KGELPSPANP PKGCVFHTRC 310 320 PVAKPICKEQ IPEFKEAAPS HFVACHLYS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a second oligopeptide transport system in Bacillus subtilis and determination of its role in sporulation." Koide A., Hoch J.A. Mol. Microbiol. 13:417-426(1994) [PubMed: 7997159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20909 Genomic DNA. Translation: AAA62357.1. Z99110 Genomic DNA. Translation: CAB12994.1.
PIR	I40544.
RefSeq	NP_389019.1.
3D structure databases
HSSP	HSSP built from PDB template 1B0U based on UniProtKB P02915.
ModBase	Search...
Genome annotation databases
GeneID	936390.
GenomeReviews	Gene locus BSU11370 in contig AL009126_GR.
KEGG	bsu:BSU11370.
NMPDR	fig|224308.1.peg.1138.
Organism-specific databases
SubtiList	BG11086. appF. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P42065.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU1138-MON.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase_core. IPR003439. ABC_transporter-like. IPR010066. Oligo_ATP_bd_C. IPR013563. Oligopep_ABC_C. [Graphical view]
Pfam	PF00005. ABC_tran. 1 hit. PF08352. oligo_HPY. 1 hit. [Graphical view]
ProDom	PD000006. ABC_transporter. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01727. oligo_HPY. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APPF_BACSU

Accession

Primary (citable) accession number: P42065

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)