Reviewed, UniProtKB/Swiss-Prot P42528 (ARP3_DICDI)

Names and origin

Protein names

Recommended name:
    Actin-related protein 3
Alternative name(s):
    Actin-like protein 3
    Actin-related protein C

Gene names

Name:	arpC
Synonyms:	aclA, act
ORF Names:	DDB_0219936

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament. The Arp2/3 complex is involved in organizing the actin system in cell motility and chemotaxis, in phagocytosis and macropinocytosis, at late steps of endosome processing, and in mitosis. In concert with a group of other proteins, the Arp2/3 complex plays a general role in the rapid activation and adaptation of the actin system to its multiple functions.
Subunit structure	Component of the Arp2/3 complex composed of arpB/Arp2, arpC/Arp3, arcA/p41-arc, arcB/p34-arc, arcC/p21-arc, arcD/p20-arc and arcE/p16-arc.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection. Cytoplasm › cytosol. Cytoplasm › cell cortex.
Induction	By starvation.
Sequence similarities	Belongs to the actin family. ARP3 subfamily.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cell projection Cytoplasm Cytoskeleton
Ligand	ATP-binding Actin-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 418	418	Actin-related protein 3

Sequences

	Sequence		Length	Mass (Da)
	P42528-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FFBC49A46CD05806	FASTA	418	46,698
	10 20 30 40 50 60 MNPASGLPAV VIDNGTGYTK MGYAGNNDPS FIIPTTIATQ SSKGKQTAAS QKKGVEDLDF 70 80 90 100 110 120 FIGDEAIANS KTYDMTNPVK HGQIENWTHM EQYWEHCVFK YLRCEPEDHY FLLTEPPLNA 130 140 150 160 170 180 PENREFTAEI MFETFNVPGL YIAVQAVLAL AASWTSKNAE KTLTGTVIDS GDGVTHVIPI 190 200 210 220 230 240 SEGYVIGSSI KHIPIAGRDI SSYVQQIMRE REPNIPPAES LEIAKRVKEQ YSYVCPDIVK 250 260 270 280 290 300 EFGKYDSEPD KWIKTINAQD SVTKKPFSYD VGYERFLGPE LFFNPEIASS DYLTPLPKVV 310 320 330 340 350 360 DDTIQSCPID CRRGLYKNIV LSGGSTMFKD FGKRLQRDVK RSVDYRIKRS EELSGGKIKA 370 380 390 400 410 VPLAVNVISH NMQRYAVWFG GSMLASTPEF YNVCHTKAQY DEIGPSICRF NTVIGGIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An actin-related protein from Dictyostelium discoideum is developmentally regulated and associated with mitochondria." Murgia I., Maciver S.K., Morandini P. FEBS Lett. 360:235-241(1995) [PubMed: 7883039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis." Insall R., Mueller-Taubenberger A., Machesky L., Koehler J., Simmeth E., Atkinson S.J., Weber I., Gerisch G. Cell Motil. Cytoskeleton 50:115-128(2001) [PubMed: 11807934] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium." Meima M.E., Weening K.E., Schaap P. Protein Expr. Purif. 53:283-288(2007) [PubMed: 17296313] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z46418 mRNA. Translation: CAA86553.1. AAFI02000057 Genomic DNA. Translation: EAL65492.1.
PIR	S48844. S69002.
RefSeq	XP_638880.1.
3D structure databases
HSSP	HSSP built from PDB template 1K8K based on UniProtKB P32391.
SMR	P42528. Positions 7-415.
ModBase	Search...
Genome annotation databases
GeneID	3390425.
KEGG	ddi:DDB_0219936.
Organism-specific databases
dictyBase	DDB0219936. arpC.
Family and domain databases
InterPro	IPR004000. Actin_like. IPR015623. Arp3. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit. PTHR11937:SF31. Arp3. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARP3_DICDI

Accession

Primary (citable) accession number: P42528
Secondary accession number(s): Q54QJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 23, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)