Reviewed, UniProtKB/Swiss-Prot P43419 (T2B6_BACSF)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme Bsp6I
      Short name=R.Bsp6I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme Bsp6I
    Endonuclease Bsp6I

Gene names

Name:

bsp6IR

Encoded on

Plasmid pXH13

Organism

Bacillus sp. (strain RFL6)

Taxonomic identifier

72577 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Recognizes the double-stranded sequence GCNGC and cleaves after C-2.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Plasmid
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 174	174	Type-2 restriction enzyme Bsp6I

Sequences

	Sequence		Length	Mass (Da)
	P43419-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9A5DC728F04DCEC6	FASTA	174	19,905
	10 20 30 40 50 60 MALHDFVKID KSRFEDAVKA YFLWKELNAL IKNSHNRGIN FPETISETLL CAAMGFELNR 70 80 90 100 110 120 GTGGDAREPK TQAIIESKAT SNWDRDTTSF SPSENFDALY FLRLNQRDDE LYIYNTGINS 130 140 150 160 170 DDLKSIQVNK TQTLGDQQAQ GRRPRFSVIK FIIEANDIEP VAKVELRTKK IIKL

References

[1]	"Cloning and analysis of the plasmid-borne genes encoding the Bsp6I restriction and modification enzymes." Lubys A., Janulaitis A. Gene 157:25-29(1995) [PubMed: 7607501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X81638 Genomic DNA. Translation: CAA57292.1.
PIR	I40137.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	485. Bsp6I.
Family and domain databases
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

T2B6_BACSF

Accession

Primary (citable) accession number: P43419

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)