Reviewed, UniProtKB/Swiss-Prot P43422 (MTV1_BACST)

Names and origin

Protein names

Recommended name:
    Modification methylase BstVI
      Short name=M.BstVI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase BstVI

Gene names

Name:

bstVIM

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BstVI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 561	561	Modification methylase BstVI

Sequences

	Sequence		Length	Mass (Da)
	P43422-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4D463062E4941859	FASTA	561	65,702
	10 20 30 40 50 60 MVTQHSYRMH GAVYTKPIIV DLILDLTGYT SDKNLENFKL LDPSFGDGVF LEAAVHRLMD 70 80 90 100 110 120 SLIRRGYRPN ELIDHLGNCI RGIELRLEAY QAGRHRLQKV LEGYGFSKPE INWLINQWII 130 140 150 160 170 180 QADFLLWQED TTEAIKFDFV VGNPPYVRQE LIQDELIKKY RKRYTTIYDR ADLYVPFIQH 190 200 210 220 230 240 SLELLSEQGT LGIICSDRFT KNRYGKKLRK FITDNYKVRY IVDLHKTSPF ENEVTAYPAI 250 260 270 280 290 300 YVIKTKNYDK SVVRAVYTEV ITSKVCQDAK DFLLSNQKPD QSSKEMKTYV FSEWFAGDEP 310 320 330 340 350 360 WIIQSQECRE ILRRLENRFP LIEDDVHSCK IRIGVATGAD KVYIVDPQQV DIEPEVLLPL 370 380 390 400 410 420 VTTADISSGR IIWSGKHVIN PFNSDGGLIN LDDFPRLKTY FQQHEEIIKN RNVAKKNPSQ 430 440 450 460 470 480 WFRTIDRIYP EIVHQPKLLI PDMKNTNHIV KDDGAFYPHH NLYYILPGNW NIDILRAILL 490 500 510 520 530 540 SSVVKFFIWS YATKMRGDTL RYQAQYLRKI RLPDPKSLTN DQKERLMDER VIQSQEYLDS 550 560 IVAEIYQLSK TEIEIIKDAL E

References

[1]	"Characterization of the bstVIRM genes encoding the Bacillus stearothermophilus V restriction-modification system." Gonzalez E., Vasquez C. Gene 131:103-106(1993) [PubMed: 8370531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: V.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L07642 Genomic DNA. Translation: AAA51408.1.
PIR	JN0797.
3D structure databases
HSSP	HSSP built from PDB template 2ADM based on UniProtKB P14385.
ModBase	Search...
Protein family/group databases
REBASE	3331. M.BstVI.
Family and domain databases
InterPro	IPR002296. N12N6_MeTrfase. IPR002052. N6_adenine_Mtase_CS. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProDom	P43422. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43422.
ProtoNet	Search...

Entry information

Entry name

MTV1_BACST

Accession

Primary (citable) accession number: P43422

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)