Reviewed, UniProtKB/Swiss-Prot P43423 (MTC1_BACST)

Names and origin

Protein names

Recommended name:
    Modification methylase BseCI
      Short name=M.BseCI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase BseCI

Gene names

Name:

bseCIM

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 579	579	Modification methylase BseCI

Sequences

	Sequence		Length	Mass (Da)
	P43423-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A4805FBEBA01835C	FASTA	579	66,774
	10 20 30 40 50 60 MMSVQKANTV SRQKATGAHF TPDKLAEVIA KRILDYFKGE KNRVIRVLDP ACGDGELLLA 70 80 90 100 110 120 INKVAQSMNI QLELIGVDFD IDAINIANER LSRSGHKNFR LINKDFLEMV SEGDNYDLFN 130 140 150 160 170 180 IEELEPVDII IANPPYVRTQ ILGAEKAQKL REKFNLKGRV DLYQAFLVAM TQQLKSNGII 190 200 210 220 230 240 GVITSNRYLT TKGGGSTRKF LVSNFNILEI MDLGDSKFFE AAVLPAIFFG EKKNKEYQKE 250 260 270 280 290 300 NSNVPKFFKI YEQSDIEASS SVNSEFNSLI ELLEVNKSGL YSVEDKTYSI SLGKIISPEN 310 320 330 340 350 360 YKEPWILATE DEYEWFMKVN QNAYGFIEDF AHVKVGIKTT ADSVFIRSDW GELPEEQIPE 370 380 390 400 410 420 DKLLRPIISA DQANKWSVSL VGNNKKVLYT HEIRDGQIKA INLEEFPRAK NYLESHKERL 430 440 450 460 470 480 ASRKYVLKAN RNWYEIWVPH DPSLWDKPKI IFPDTSPEPK FFYEDKGSVV DGNCYWIIPK 490 500 510 520 530 540 KENSNDILFL IMGICNSKFM SKYHDIAFQN KLYAGRRRYL TQYVNKYPIP DPESIYSKEI 550 560 570 ISLVRELVNN KKETQDINEI ENRIEKLILR AFDIESLKY

References

[1]	"Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII." Rina M., Markaki M., Bouriotis V. Gene 150:71-73(1994) [PubMed: 7959066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79509 Genomic DNA. Translation: CAA56041.1.
PIR	I40371.
3D structure databases
HSSP	HSSP built from PDB template 2ADM based on UniProtKB P14385.
ModBase	Search...
Protein family/group databases
REBASE	3536. M.BseCI.
Family and domain databases
InterPro	IPR002296. N12N6_MeTrfase. IPR002052. N6_adenine_Mtase_CS. IPR003356. N6_DNA_Mtase. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProDom	P43423. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MTC1_BACST

Accession

Primary (citable) accession number: P43423

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)