Reviewed, UniProtKB/Swiss-Prot P43542 (COS4_YEAST)

Names and origin

Protein names

Recommended name:
    Protein COS4

Gene names

Name:	COS4
Ordered Locus Names:	YFL062W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane proteinPotential.
Sequence similarities	Belongs to the DUP/COS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 379	379	Protein COS4
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	70 – 90	21	Potential
	Transmembrane	233 – 253	21	Potential
	Transmembrane	255 – 275	21	Potential
Amino acid modifications
	Cross-link	205		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Sequences

	Sequence		Length	Mass (Da)
	P43542-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6822561BC9890275	FASTA	379	45,312
	10 20 30 40 50 60 MKENELKNEK SVDVLSFKQL ESQKIVLPQD LFRSSFTWFC YEIYKSLAFR IWMLLWLPLS 70 80 90 100 110 120 VWWKLSNNWI YPLMVSLLVL FWGPVFVLVI FRLSRKRSLS KQLTQFCKEI TKSTPSSDPH 130 140 150 160 170 180 DWEVVAANLN SYLYENKAWN IRYFFFNAMG CQEAFRTTLL EPFSLKKDEA AKVKSFKDSV 190 200 210 220 230 240 PYIEEALGVY FREVEKQWKL FNSEKSWSPV GLEDAKLPKE AYRFKLTWFL KRISNIFMLI 250 260 270 280 290 300 PFLNFLCCIY VSRGMCLLLR TLYLGWILFM LVQGFQNIRV LIMSMEHKMQ FLSTIINEQE 310 320 330 340 350 360 SGANGWDEIA RKMNRYLFEK KVWKNEEFFF DGIDCEWFFS HFFYRVLSAK KSMRALSLNV 370 ELWPYIKEAQ LSCSEESLA

References

[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-205, MASS SPECTROMETRY.
[3]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed: 14557538] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-205, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09179.1.
PIR	S56193.
RefSeq	NP_116593.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7524N.
Genome annotation databases
Ensembl	YFL062W. Saccharomyces cerevisiae. [Contig view]
GeneID	850482.
GenomeReviews	Gene locus YFL062W in contig D50617_GR.
KEGG	sce:YFL062W.
NMPDR	fig|4932.3.peg.2216.
Organism-specific databases
CYGD	YFL062w.
SGD	S000001832. COS4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43542.
Gene expression databases
GermOnline	YFL062W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001142. DUP. [Graphical view]
Pfam	PF00674. DUP. 2 hits. [Graphical view]
ProDom	P43542. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43542.
ProtoNet	Search...

Entry information

Entry name

COS4_YEAST

Accession

Primary (citable) accession number: P43542

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)