Reviewed, UniProtKB/Swiss-Prot P43548 (AGP3_YEAST)

Names and origin

Protein names

Recommended name:
    General amino acid permease AGP3

Gene names

Name:	AGP3
Ordered Locus Names:	YFL055W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	General amino acid permease with broad substrate specificity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from direct assay. Source: SGD negative regulation of transposition, RNA-mediated Inferred from mutant phenotype. Source: SGD
Molecular function	amino acid transmembrane transporter activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 558	558	General amino acid permease AGP3
Regions
	Topological domain	1 – 57	57	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Potential
	Topological domain	79 – 81	3	Extracellular Potential
	Transmembrane	82 – 102	21	Potential
	Topological domain	103 – 126	24	Cytoplasmic Potential
	Transmembrane	127 – 147	21	Potential
	Topological domain	148 – 168	21	Extracellular Potential
	Transmembrane	169 – 189	21	Potential
	Topological domain	190 – 193	4	Cytoplasmic Potential
	Transmembrane	194 – 214	21	Potential
	Topological domain	215 – 241	27	Extracellular Potential
	Transmembrane	242 – 262	21	Potential
	Topological domain	263 – 280	18	Cytoplasmic Potential
	Transmembrane	281 – 301	21	Potential
	Topological domain	302 – 324	23	Extracellular Potential
	Transmembrane	325 – 345	21	Potential
	Topological domain	346 – 375	30	Cytoplasmic Potential
	Transmembrane	376 – 396	21	Potential
	Topological domain	397 – 402	6	Extracellular Potential
	Transmembrane	403 – 423	21	Potential
	Topological domain	424 – 446	23	Cytoplasmic Potential
	Transmembrane	447 – 467	21	Potential
	Topological domain	468 – 480	13	Extracellular Potential
	Transmembrane	481 – 501	21	Potential
	Topological domain	502 – 558	57	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P43548-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D7AE03BE36ADA1A1	FASTA	558	61,052
	10 20 30 40 50 60 MAVLNLKRET VDIEETAKKD IKPYFASNVE AVDIDEDPDV SRYDPQTGVK RALKNRHISL 70 80 90 100 110 120 LALGGVIGPG CLVGAGNALN KGGPLALLLG FSIIGIIAFS VMESIGEMIT LYPSGGGFTT 130 140 150 160 170 180 LARRFHSDAL PAVCGYAYVV VFFAVLANEY NTLSSILQFW GPQVPLYGYI LIFWFAFEIF 190 200 210 220 230 240 QLVGVGLFGE TEYWLAWLKI VGLVAYYIFS IVYISGDIRN RPAFGFHYWN SPGALSHGFK 250 260 270 280 290 300 GIAIVFVFCS TFYSGTESVA LAATESKNPG KAVPLAVRQT LWRILVVYIG IAVFYGATVP 310 320 330 340 350 360 FDDPNLSAST KVLKSPIAIA ISRAGWAGGA HLVNAFILIT CISAINGSLY IGSRTLTHLA 370 380 390 400 410 420 HEGLAPKILA WTDRRGVPIP AITVFNALGL ISLMNVSVGA ANAYSYIVNL SGVGVFIVWG 430 440 450 460 470 480 VISYTHLRIR KAWVAQGRSI EELPYEALFY PWTPVLSLAA NIFLALIQGW SYFVPFDAGN 490 500 510 520 530 540 FVDAYILLPV GILLYIGICV FKSNHFRTVD LRSINLDEGR RKDMEADLSD QESSLASSET 550 MKDYKSATFF RYLSNIFT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Garrett J.M., Schreve J.L. Unpublished observations (JUL-1997) Cited for: CHARACTERIZATION.
[3]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09186.1.
PIR	S56200.
RefSeq	NP_116600.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4160N.
Genome annotation databases
Ensembl	YFL055W. Saccharomyces cerevisiae. [Contig view]
GeneID	850489.
GenomeReviews	Gene locus YFL055W in contig D50617_GR.
KEGG	sce:YFL055W.
NMPDR	fig|4932.3.peg.2223.
Organism-specific databases
CYGD	YFL055w.
SGD	S000001839. AGP3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43548.
Gene expression databases
ArrayExpress	P43548.
GermOnline	YFL055W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004762. Amino_acid_permease_fungi. IPR004840. Amoino_acid_permease_CS. IPR004841. Permease_region. [Graphical view]
PANTHER	PTHR11785. AA/rel_permease1. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00913. 2A0310. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProDom	P43548. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43548.
ProtoNet	Search...

Entry information

Entry name

AGP3_YEAST

Accession

Primary (citable) accession number: P43548

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)