Reviewed, UniProtKB/Swiss-Prot P43555 (EMP47_YEAST)

Names and origin

Protein names

Recommended name:
    Protein EMP47
Alternative name(s):
    47 kDa endomembrane protein
    Endosomal P44 protein

Gene names

Name:	EMP47
Ordered Locus Names:	YFL048C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46.
Subunit structure	Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatamer proteins COP1, SEC21 and SEC23.
Subcellular location	Golgi apparatus membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Domain	The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the EMP46/EMP47 family. Contains 1 L-type lectin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Signal Transmembrane
Ligand	Lectin
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from physical interaction. Source: SGD
Cellular component	ER to Golgi transport vesicle Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Signal peptide

1 – 28

28

Chain

29 – 445

417

Protein EMP47

Regions

Topological domain

29 – 412

384

Lumenal Potential

Transmembrane

413 – 433

21

Potential

Topological domain

434 – 445

12

Cytoplasmic Potential

Domain

36 – 254

219

L-type lectin-like

Region

430 – 433

4

Mediates the interactions with COPI and COPII coat complexes By similarity

Motif

441 – 445

5

Di-lysine motif

Amino acid modifications

Disulfide bond

179 ↔ 213

Experimental info

Sequence conflict

52

1

A → T in CAA60953. Ref.1

Sequence conflict

77

1

K → N in CAA60953. Ref.1

Sequence conflict

125

1

V → I in CAA60953. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

445

Helix Strand Turn

Details...

Turn

37 – 39

3

Helix

42 – 44

3

Beta strand

59 – 63

5

Beta strand

66 – 68

3

Beta strand

70 – 73

4

Beta strand

81 – 88

8

Beta strand

90 – 94

5

Beta strand

96 – 106

11

Beta strand

114 – 121

8

Beta strand

123 – 125

3

Beta strand

139 – 147

9

Beta strand

153 – 164

12

Helix

168 – 170

3

Helix

171 – 174

4

Beta strand

176 – 180

5

Beta strand

190 – 197

8

Helix

198 – 200

3

Beta strand

201 – 208

8

Beta strand

211 – 217

7

Beta strand

223 – 233

11

Helix

237 – 239

3

Beta strand

243 – 254

12

Sequences

	Sequence		Length	Mass (Da)
	P43555-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 3978462BC2AFCD8A	FASTA	445	50,330
	10 20 30 40 50 60 MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI NARKVPNNWQ 70 80 90 100 110 120 TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW TFRSVGYSGQ TDGGISFWFV 130 140 150 160 170 180 QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP LGPTLRGQLN DGQKPVDKTK IYDQSFASCL 190 200 210 220 230 240 MGYQDSSVPS TIRVTYDLED DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN 250 260 270 280 290 300 AESFEIFKMQ FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT 310 320 330 340 350 360 NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS KPPANNEKGT 370 380 390 400 410 420 STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH GQDGPQVDEI ARKLMIWLLP 430 440 LIFIMLVMAY YTFRIRQEII KTKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Golgi-localization of yeast Emp47p depends on its di-lysine motif but is not affected by the ret1-1 mutation in alpha-COP." Schroeder S., Schimmoeller F., Singer-Krueger B., Riezman H. J. Cell Biol. 131:895-912(1995) [PubMed: 7490292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Partial purification and characterization of early and late endosomes from yeast. Identification of four novel proteins." Singer-Krueger B., Frank R., Crausaz F., Riezman H. J. Biol. Chem. 268:14376-14386(1993) [PubMed: 8314797] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-40. Strain: RH732.
[5]	"Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae." Sato K., Nakano A. Mol. Biol. Cell 13:2518-2532(2002) [PubMed: 12134087] [Abstract] Cited for: FUNCTION, DOMAINS.
[6]	"Oligomerization of a cargo receptor directs protein sorting into COPII-coated transport vesicles." Sato K., Nakano A. Mol. Biol. Cell 14:3055-3063(2003) [PubMed: 12857885] [Abstract] Cited for: INTERACTION WITH EMP46, SUBUNIT, SUBCELLULAR LOCATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Reconstitution of coat protein complex II (COPII) vesicle formation from cargo-reconstituted proteoliposomes reveals the potential role of GTP hydrolysis by Sar1p in protein sorting." Sato K., Nakano A. J. Biol. Chem. 279:1330-1335(2004) [PubMed: 14627716] [Abstract] Cited for: FUNCTION.
[9]	"Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p." Satoh T., Sato K., Kanoh A., Yamashita K., Yamada Y., Igarashi N., Kato R., Nakano A., Wakatsuki S. J. Biol. Chem. 281:10410-10419(2006) [PubMed: 16439369] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1 ANGSTROMS) OF 29-282.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X87622 Genomic DNA. Translation: CAA60953.1.
D50617 Genomic DNA. Translation: BAA09193.1.
AY693033 Genomic DNA. Translation: AAT93052.1.

PIR

S56207.

RefSeq

NP_116606.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A6Y	X-ray	1.42	A	29-282	[»]
2A6Z	X-ray	1.00	A	35-255	[»]
2A70	X-ray	1.10	A/B	35-255	[»]
2A71	X-ray	2.70	A/B/C/D	35-255	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:7675N.

IntAct

P43555.

Proteomic databases

PeptideAtlas

P43555.

Genome annotation databases

Ensembl

YFL048C. Saccharomyces cerevisiae. [Contig view]

GeneID

850496.

GenomeReviews

Gene locus YFL048C in contig D50617_GR.

KEGG

sce:YFL048C.

NMPDR

fig|4932.3.peg.2230.

Organism-specific databases

CYGD

YFL048c.

SGD

S000001846. EMP47.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P43555.

Gene expression databases

ArrayExpress

P43555.

GermOnline

YFL048C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013320. ConA_like_subgrp.
IPR016710. L-typ_lectin_fun.
IPR005052. Lectin_leg.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

PANTHER

PTHR12223. Lectin_leg. 1 hit.

Pfam

PF03388. Lectin_leg-like. 1 hit.
[Graphical view]

PIRSF

PIRSF018136. L-type_lectin_fungi. 1 hit.

PROSITE

PS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

ProDom

P43555.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P43555.

ProtoNet

Search...

Entry information

Entry name

EMP47_YEAST

Accession

Primary (citable) accession number: P43555

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)