Reviewed, UniProtKB/Swiss-Prot P43558 (OTU1_YEAST)

Names and origin

Protein names

Recommended name:
    Ubiquitin thioesterase OTU1
    EC=3.1.2.-
Alternative name(s):
    OTU domain-containing protein 1

Gene names

Name:	OTU1
Ordered Locus Names:	YFL044C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Participates in the regulation of the ubiquin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May be indireclty involved in PIS1 gene expression.
Subunit structure	Interacts with CDC48 and DOA1/UFD3.
Subcellular location	Cytoplasm. Nucleus.
Domain	The OTU domain mediates the hydrolase activity and the interaction with CDC48.
Miscellaneous	Present with 2770 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 C2H2-type zinc finger. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein deubiquitination Ref.5 Inferred from direct assay. Source: SGD regulation of transcription Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.2 Inferred from direct assay. Source: SGD nucleus Ref.2 Inferred from direct assay. Source: SGD
Molecular function	ubiquitin-specific protease activity Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 301	301	Ubiquitin thioesterase OTU1
Regions
	Domain	109 – 229	121	OTU
	Zinc finger	270 – 294	25	C2H2-type
Sites
	Active site	117	1	By similarity
	Active site	120	1
	Active site	294	1	By similarity
Experimental info
	Mutagenesis	120	1	C → S: Loss of function

Sequences

	Sequence		Length	Mass (Da)
	P43558-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 32ABA3CFEA4BB5EB	FASTA	301	33,510
	10 20 30 40 50 60 MKLKVTGAGI NQVVTLKQDA TLNDLIEHIN VDVKTMRFGY PPQRINLQGE DASLGQTQLD 70 80 90 100 110 120 ELGINSGEKI TIESSDSNES FSLPPPQPKP KRVLKSTEMS IGGSGENVLS VHPVLDDNSC 130 140 150 160 170 180 LFHAIAYGIF KQDSVRDLRE MVSKEVLNNP VKFNDAILDK PNKDYAQWIL KMESWGGAIE 190 200 210 220 230 240 IGIISDALAV AIYVVDIDAV KIEKFNEDKF DNYILILFNG IHYDSLTMNE FKTVFNKNQP 250 260 270 280 290 300 ESDDVLTAAL QLASNLKQTG YSFNTHKAQI KCNTCQMTFV GEREVARHAE STGHVDFGQN R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Genomic analysis of PIS1 gene expression." Gardocki M.E., Bakewell M., Kamath D., Robinson K., Borovicka K., Lopes J.M. Eukaryot. Cell 4:604-614(2005) [PubMed: 15755922] [Abstract] Cited for: FUNCTION.
[5]	"Functional division of substrate processing cofactors of the ubiquitin-selective Cdc48 chaperone." Rumpf S., Jentsch S. Mol. Cell 21:261-269(2006) [PubMed: 16427015] [Abstract] Cited for: FUNCTION, DOMAIN OTU, INTERACTION WITH CDC48 AND DOA1, MUTAGENESIS OF CYS-120.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D50617 Genomic DNA. Translation: BAA09197.1.

PIR

S56211.

RefSeq

NP_116610.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BY4	X-ray	1.55	A	91-301	[»]
3C0R	X-ray	2.31	A/C	91-301	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4672N.

IntAct

P43558.

Protein family/group databases

MEROPS

C88.001.

Proteomic databases

PeptideAtlas

P43558.

Genome annotation databases

Ensembl

YFL044C. Saccharomyces cerevisiae. [Contig view]

GeneID

850500.

GenomeReviews

Gene locus YFL044C in contig D50617_GR.

KEGG

sce:YFL044C.

NMPDR

fig|4932.3.peg.2234.

Organism-specific databases

CYGD

YFL044c.

SGD

S000001850. OTU1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P43558.

Gene expression databases

GermOnline

YFL044C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003323. OTU.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]

Pfam

PF02338. OTU. 1 hit.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 1 hit.
[Graphical view]

PROSITE

PS50802. OTU. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. False negative.
[Graphical view]

ProDom

P43558.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

LinkHub

P43558.

ProtoNet

Search...

Entry information

Entry name

OTU1_YEAST

Accession

Primary (citable) accession number: P43558

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)