Reviewed, UniProtKB/Swiss-Prot P43563 (MOB2_YEAST)

Names and origin

Protein names

Recommended name:
    CBK1 kinase activator protein MOB2
Alternative name(s):
    Maintenance of ploidy protein MOB2
    MPS1 binder 2

Gene names

Name:	MOB2
Ordered Locus Names:	YFL034C-B
ORF Names:	YFL035C, YFL035C-A

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions as an activator subunit for the CBK1 protein kinase. Part of the regulation of ACE2 activity and cellular morphogenesis (RAM) signaling network. Required for coordinating polarized cell growth during interphase with the onset of mitosis. Required for mother/daughter cell separation after cytokinesis. Also has a role in the prevention of nuclear export of ACE2 from the daughter cell nucleus after mitotic exit. It coordinates ACE2-dependent transcription with mitotic exit network activation.
Subunit structure	Interacts with protein kinase CBK1 to form the RAM CBK1-MOB2 kinase complex.
Subcellular location	Nucleus. Cytoplasm. Note= Localizes to the bud cortex during polarized growth and to the bud neck and daughter cell nucleus during late mitosis.
Miscellaneous	Present with 2250 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MOB1/phocein family.
Sequence caution	The sequence BAA09204.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Nucleus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular morphogenesis during vegetative growth Inferred from genetic interaction. Source: SGD establishment or maintenance of cell polarity Ref.4 Inferred from mutant phenotype. Source: SGD protein amino acid phosphorylation Ref.4 Inferred from direct assay. Source: SGD regulation of exit from mitosis Ref.4 Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud neck Ref.4 Inferred from direct assay. Source: SGD mating projection tip Traceable author statement. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein kinase activator activity Ref.4 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P40572	1	EBI-11125,EBI-25414
DID4	P36108	1	EBI-11125,EBI-26574

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 287	287	CBK1 kinase activator protein MOB2

Sequences

	Sequence		Length	Mass (Da)
	P43563-1 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: CFBA15FF83BFAA33	FASTA	287	33,276
	10 20 30 40 50 60 MSFFNFKAFG RNSKKNKNQP LNVAQPPAMN TIYSSPHSSN SRLSLRNKHH SPKRHSQTSF 70 80 90 100 110 120 PAQKSTPQSQ QLTSTTPQSQ QQEASERSES QQIMFLSEPF VRTALVKGSF KTIVQLPKYV 130 140 150 160 170 180 DLGEWIALNV FEFFTNLNQF YGVVAEYVTP DAYPTMNAGP HTDYLWLDAN NRQVSLPASQ 190 200 210 220 230 240 YIDLALTWIN NKVNDKNLFP TKNGLPFPQQ FSRDVQRIMV QMFRIFAHIY HHHFDKIVHL 250 260 270 280 SLEAHWNSFF SHFISFAKEF KIIDRKEMAP LLPLIESFEK QGKIIYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy." Luca F.C., Winey M. Mol. Biol. Cell 9:29-46(1998) [PubMed: 9436989] [Abstract] Cited for: IDENTIFICATION OF INTRON.
[3]	"Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates." Colman-Lerner A., Chin T.E., Brent R. Cell 107:739-750(2001) [PubMed: 11747810] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]	"The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor." Weiss E.L., Kurischko C., Zhang C., Shokat K., Drubin D.G., Luca F.C. J. Cell Biol. 158:885-900(2002) [PubMed: 12196508] [Abstract] Cited for: FUNCTION, INTERACTION WITH CBK1, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Structural and functional analysis of Saccharomyces cerevisiae Mob1." Mrkobrada S., Boucher L., Ceccarelli D.F.J., Tyers M., Sicheri F. J. Mol. Biol. 362:430-440(2006) [PubMed: 16934835] [Abstract] Cited for: INTERACTION WITH MOB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09204.1. Sequence problems.
PIR	S58648.
RefSeq	NP_116618.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1449N.
IntAct	P43563.
Genome annotation databases
Ensembl	YFL034C-B. Saccharomyces cerevisiae. [Contig view]
GeneID	850508.
GenomeReviews	Gene locus YFL034C-B in contig D50617_GR.
KEGG	sce:YFL034C-B.
NMPDR	fig|4932.3.peg.2243.
Organism-specific databases
CYGD	YFL034c-b.
SGD	S000001859. MOB2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43563.
Gene expression databases
GermOnline	YFL034C-B. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005301. Mob1_phocein. [Graphical view]
Gene3D	G3DSA:1.20.140.30. Mob1_phocein. 1 hit.
PANTHER	PTHR22599. Mob1_phocein. 1 hit.
Pfam	PF03637. Mob1_phocein. 1 hit. [Graphical view]
ProDom	P43563. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43563.
ProtoNet	Search...

Entry information

Entry name

MOB2_YEAST

Accession

Primary (citable) accession number: P43563

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 17, 2006
Last modified:	July 22, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)