Reviewed, UniProtKB/Swiss-Prot P43573 (BUD27_YEAST)

Names and origin

Protein names

Recommended name:
    Bud site selection protein 27

Gene names

Name:	BUD27
Synonyms:	URI
Ordered Locus Names:	YFL023W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in gene expression controlled by TOR kinase and nutrient signaling. May also be involved in positioning the proximal bud pole signal.
Subcellular location	Cytoplasm.
Miscellaneous	Present with 1300 molecules/cell in log phase SD medium. Deletion leads to a K1 killer toxin hypersensitivity.
Sequence similarities	Belongs to the prefoldin subunit alpha family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular bud site selection Ref.2 Inferred from mutant phenotype. Source: SGD negative regulation of transposition, RNA-mediated Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
FPR1	P20081	1	EBI-22787,EBI-6961
RPB5	P20434	1	EBI-22787,EBI-15781
SSB1	P11484	1	EBI-22787,EBI-8627
TEF1	P02994	1	EBI-22787,EBI-6314
TEF4	P36008	1	EBI-22787,EBI-6329
YKE2	P52553	1	EBI-22787,EBI-13260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 796	796	Bud site selection protein 27
Regions
	Coiled coil	81 – 121	41	Potential
Amino acid modifications
	Modified residue	580	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P43573-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7E2EE2CEB4708BC3	FASTA	796	91,422
	10 20 30 40 50 60 MDLLAASVES TLKNLQDKRN FLSEQREHYI DIRSRLVRFI NDNDDGEEEG EGQGMVFGDI 70 80 90 100 110 120 IISTSKIYLS LGYEYYVEKT KEEAITFVDD KLKLMEDAIE QFNLKIEEAK KTLDNLNHME 130 140 150 160 170 180 DGNGIEEDEA NNDEDFLPSM EIREELDDEG NVISSSVTPT TKQPSQSNSK KEQTPAVGPK 190 200 210 220 230 240 EKGLAKEKKS KSFEENLKGK LLKRNDEVKK KVQPSKVDTE NVYTFADLVQ QMDQQDELED 250 260 270 280 290 300 GYIETDEINY DYDAFENSNF KVNDNYEEDD EDEDEEEYLN HSIIPGFEAQ SSFLQQIQRL 310 320 330 340 350 360 RAQKQSQDHE REEGDVNKSL KPILKKSSFA ENSDKKQKKK QVGFASSLEI HEVENLKEEN 370 380 390 400 410 420 KRQMQSFAVP MYETQESTGI ANKMTSDEFD GDLFAKMLGV QEADEVHEKY KEELINQERL 430 440 450 460 470 480 EGEASRSNRR TRVSRFRKDR ASKKENTLST FKQETTRSVE NEVVEKEPVV GDIIEKEPVV 490 500 510 520 530 540 GDVIEKEPVV GDVIEKEPAV TDIVEREPAV NDIVERKPVV GDIIEKEPTI NDIVEKEPEI 550 560 570 580 590 600 NSKSEFETPF KKKKLKSLQK PRSSKSMKKK FDPKILENIS DDDYDDDDDG NKKLLSNKSK 610 620 630 640 650 660 NNTDEQDKFP SKIQEVSRSM AKTGATVGSE PVRITNVDYH ALGGNLDDMV KAYSLGLYDD 670 680 690 700 710 720 DLEEDPGTIV EKLEDFKEYN KQVELLRDEI RDFQLENKPV TMEEEENDGN VMNDIIEHEF 730 740 750 760 770 780 PESYTNDEDE VALHPGRLQE EVAIEYRRLK EATASKWQSS SPAAHTEGEL EPIDKFGNPV 790 KTSRFRSQRL HMDSKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: FUNCTION.
[3]	"A Saccharomyces cerevisiae genome-wide mutant screen for altered sensitivity to K1 killer toxin." Page N., Gerard-Vincent M., Menard P., Beaulieu M., Azuma M., Dijkgraaf G.J.P., Li H., Marcoux J., Nguyen T., Dowse T., Sdicu A.-M., Bussey H. Genetics 163:875-894(2003) [PubMed: 12663529] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI." Gstaiger M., Luke B., Hess D., Oakeley E.J., Wirbelauer C., Blondel M., Vigneron M., Peter M., Krek W. Science 302:1208-1212(2003) [PubMed: 14615539] [Abstract] Cited for: FUNCTION.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-580, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09215.1.
PIR	S56231.
RefSeq	NP_683715.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4929N.
IntAct	P43573.
Proteomic databases
PeptideAtlas	P43573.
Genome annotation databases
Ensembl	YFL023W. Saccharomyces cerevisiae. [Contig view]
GeneID	850521.
GenomeReviews	Gene locus YFL023W in contig D50617_GR.
KEGG	sce:YFL023W.
NMPDR	fig|4932.3.peg.2257.
Organism-specific databases
CYGD	YFL023w.
SGD	S000001871. BUD27.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43573.
Gene expression databases
ArrayExpress	P43573.
GermOnline	YFL023W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004127. PFD_alpha_like. [Graphical view]
Pfam	PF02996. Prefoldin. 1 hit. [Graphical view]
ProDom	P43573. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43573.
ProtoNet	Search...

Entry information

Entry name

BUD27_YEAST

Accession

Primary (citable) accession number: P43573

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)