Reviewed, UniProtKB/Swiss-Prot P43586 (LOC1_YEAST)

Names and origin

Protein names

Recommended name:
    60S ribosomal subunit assembly/export protein LOC1
Alternative name(s):
    Localization of ASH1 mRNA protein 1

Gene names

Name:	LOC1
Ordered Locus Names:	YFR001W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for efficient assembly and nuclear export of the 60S ribosomal subunit. Involved in asymmetric localization of ASH1 mRNA.
Subunit structure	Component of the 66S pre-ribosomal particle. Interacts with NOP7, RRP1 and RRP15.
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 3650 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the LOC1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transport mRNA transport
Cellular component	Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular mRNA localization Ref.4 Inferred from mutant phenotype. Source: SGD ribosomal large subunit biogenesis Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	mRNA binding Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ISU1	Q03020	1	EBI-22906,EBI-29901

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 204	204	60S ribosomal subunit assembly/export protein LOC1
Regions
	Coiled coil	120 – 166	47	Potential
	Compositional bias	39 – 199	161	Lys-rich

Sequences

	Sequence		Length	Mass (Da)
	P43586-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 69970294E9C980F9	FASTA	204	23,590
	10 20 30 40 50 60 MAPKKPSKRQ NLRREVAPEV FQDSQARNQL ANVPHLTEKS AQRKPSKTKV KKEQSLARLY 70 80 90 100 110 120 GAKKDKKGKY SEKDLNIPTL NRAIVPGVKI RRGKKGKKFI ADNDTLTLNR LITTIGDKYD 130 140 150 160 170 180 DIAESKLEKA RRLEEIRELK RKEIERKEAL KQDKLEEKKD EIKKKSSVAR TIRRKNKRDM 190 200 LKSEAKASES KTEGRKVKKV SFAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast." Long R.M., Gu W., Meng X., Gonsalvez G.B., Singer R.H., Chartrand P. J. Cell Biol. 153:307-318(2001) [PubMed: 11309412] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Composition and functional characterization of yeast 66S ribosome assembly intermediates." Harnpicharnchai P., Jakovljevic J., Horsey E., Miles T., Roman J., Rout M., Meagher D., Imai B., Guo Y., Brame C.J., Shabanowitz J., Hunt D.F., Woolford J.L. Jr. Mol. Cell 8:505-515(2001) [PubMed: 11583614] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH NOP7.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Role of the yeast Rrp1 protein in the dynamics of pre-ribosome maturation." Horsey E.W., Jakovljevic J., Miles T.D., Harnpicharnchai P., Woolford J.L. Jr. RNA 10:813-827(2004) [PubMed: 15100437] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP1.
[9]	"Rrp15p, a novel component of pre-ribosomal particles required for 60S ribosome subunit maturation." De Marchis M.L., Giorgi A., Schinina M.E., Bozzoni I., Fatica A. RNA 11:495-502(2005) [PubMed: 15769876] [Abstract] Cited for: MASS SPECTROMETRY, INTERACTION WITH RRP15.
[10]	"Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit." Urbinati C.R., Gonsalvez G.B., Aris J.P., Long R.M. Mol. Genet. Genomics 276:369-377(2006) [PubMed: 16871394] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09240.1. AY557809 Genomic DNA. Translation: AAS56135.1.
PIR	S56256.
RefSeq	NP_116656.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6615N.
IntAct	P43586.
Proteomic databases
PeptideAtlas	P43586.
Genome annotation databases
Ensembl	YFR001W. Saccharomyces cerevisiae. [Contig view]
GeneID	850551.
GenomeReviews	Gene locus YFR001W in contig D50617_GR.
KEGG	sce:YFR001W.
NMPDR	fig|4932.3.peg.2287.
Organism-specific databases
CYGD	YFR001w.
SGD	S000001897. LOC1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43586.
Gene expression databases
GermOnline	YFR001W. Saccharomyces cerevisiae.
Family and domain databases
ProDom	P43586. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43586.
ProtoNet	Search...

Entry information

Entry name

LOC1_YEAST

Accession

Primary (citable) accession number: P43586

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)