Reviewed, UniProtKB/Swiss-Prot P43587 (YPI1_YEAST)

Names and origin

Protein names

Recommended name:
    Type 1 phosphatases regulator YPI1

Gene names

Name:	YPI1
Ordered Locus Names:	YFR003C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control. Regulates the nuclear localization of type 1 phosphatase GLC7 and SDS22, and is involved in the regulation of mRNA 3'-end processing. May also regulate the activity of type 1 phosphatase PPZ1.
Subunit structure	Interacts with GLC7, PPZ1 and SDS22.
Subcellular location	Nucleus.
Miscellaneous	Present with 1080 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the YPI1 family.
Caution	Was originally (Ref.3) thought to be an inhibitor of type 1 phosphatases using in vitro experiments, but further in vivo experiments (Ref.11) showed that it was rather an activator of these phosphatases.

Ontologies

Keywords
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen metabolic process Ref.3 Inferred from mutant phenotype. Source: SGD protein amino acid dephosphorylation Ref.3 Inferred from direct assay. Source: SGD
Cellular component	nucleus Ref.7 Inferred from direct assay. Source: SGD
Molecular function	protein phosphatase inhibitor activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 155	155	Type 1 phosphatases regulator YPI1
Regions
	Motif	51 – 53	3	GLC7-binding
	Compositional bias	93 – 103	11	Poly-Ser
Amino acid modifications
	Modified residue	20	1	Phosphothreonine
	Modified residue	22	1	Phosphoserine
	Modified residue	131	1	Phosphoserine
	Modified residue	132	1	Phosphotyrosine
	Modified residue	133	1	Phosphoserine
	Modified residue	137	1	Phosphotyrosine
Experimental info
	Mutagenesis	51	1	V → A: Prevents interaction with GLC7
	Mutagenesis	53	1	W → A: Prevents interaction with GLC7 and reduces nuclear levels of GLC7 and SDS22

Sequences

	Sequence		Length	Mass (Da)
	P43587-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E191184DCD5EF8A1	FASTA	155	18,187
	10 20 30 40 50 60 MSGNQMAMGS EQQQTVGSRT VSVEEVPAVL QLRATQDPPR SQEAMPTRHN VRWEENVIDN 70 80 90 100 110 120 ENMNKKKTKI CCIFHPQNED EEECNHHSDD DGSSSSGSSS SESENEKDLD FNERRQRRLE 130 140 150 RRHRKLEKKR SYSPNAYEIQ PDYSEYRRKQ QEKKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Molecular characterization of Ypi1, a novel Saccharomyces cerevisiae type 1 protein phosphatase inhibitor." Garcia-Gimeno M.A., Munoz I., Arino J., Sanz P. J. Biol. Chem. 278:47744-47752(2003) [PubMed: 14506263] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TRP-53, INTERACTION WITH GLC7 AND PPZ1.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Regulation of yeast mRNA 3' end processing by phosphorylation." He X., Moore C. Mol. Cell 19:619-629(2005) [PubMed: 16137619] [Abstract] Cited for: FUNCTION.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-133, MASS SPECTROMETRY.
[7]	"YPI1 and SDS22 proteins regulate the nuclear localization and function of yeast type 1 phosphatase Glc7." Pedelini L., Marquina M., Arino J., Casamayor A., Sanz L., Bollen M., Sanz P., Garcia-Gimeno M.A. J. Biol. Chem. 282:3282-3292(2007) [PubMed: 17142459] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLC7 AND SDS22.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY.
[9]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-133, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131 AND SER-133, MASS SPECTROMETRY.
[11]	"Ypi1, a positive regulator of nuclear protein phosphatase type 1 activity in Saccharomyces cerevisiae." Bharucha J.P., Larson J.R., Gao L., Daves L.K., Tatchell K. Mol. Biol. Cell 19:1032-1045(2008) [PubMed: 18172024] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH GLC7, MUTAGENESIS OF VAL-51 AND TRP-53.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-20; SER-22; SER-131; TYR-132; SER-133 AND TYR-137, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50617 Genomic DNA. Translation: BAA09242.1. AY557803 Genomic DNA. Translation: AAS56129.1.
PIR	S56258.
RefSeq	NP_116658.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5442N.
IntAct	P43587.
Genome annotation databases
Ensembl	YFR003C. Saccharomyces cerevisiae. [Contig view]
GeneID	850553.
GenomeReviews	Gene locus YFR003C in contig D50617_GR.
KEGG	sce:YFR003C.
NMPDR	fig|4932.3.peg.2289.
Organism-specific databases
CYGD	YFR003c.
SGD	S000001899. YPI1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P43587.
Gene expression databases
GermOnline	YFR003C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011107. PPI_Ypi1. [Graphical view]
PANTHER	PTHR20835. PPI_Ypi1. 1 hit.
Pfam	PF07491. PPI_Ypi1. 1 hit. [Graphical view]
ProDom	P43587. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P43587.
ProtoNet	Search...

Entry information

Entry name

YPI1_YEAST

Accession

Primary (citable) accession number: P43587

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)