Reviewed, UniProtKB/Swiss-Prot P44451 (FDXI_HAEIN)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase, cytochrome b556 subunit
Alternative name(s):
    Formate dehydrogenase subunit gamma
      Short name=FDH subunit gamma

Gene names

Name:	fdxI
Ordered Locus Names:	HI0008

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Allows to use formate as major electron donor during anaerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase By similarity.
Cofactor	Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme By similarity.
Subunit structure	Formate dehydrogenase is a membrane-bound complex, formed by subunits alpha, beta and gamma.
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Sequence similarities	Belongs to the formate dehydrogenase gamma subunit family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular respiration Inferred from electronic annotation. Source: InterPro respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	formate dehydrogenase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Formate dehydrogenase, cytochrome b556 subunit		PRO_0000087217
Regions
	Transmembrane	23 – 43	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	120 – 140	21	Potential
	Transmembrane	155 – 175	21	Potential
Sites
	Metal binding	23	1	Iron (heme B 1 axial ligand) By similarity
	Metal binding	62	1	Iron (heme B 2 axial ligand) By similarity
	Metal binding	160	1	Iron (heme B 2 axial ligand) By similarity
	Metal binding	174	1	Iron (heme B 1 axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44451-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 4D0744AA342066A2	FASTA	238	27,758
	10 20 30 40 50 60 MSKIEISNDT RIIRHRTPAR ISHWMLVICF FMTMFTGVAF FFPDFAWLTE ILGTPQIARA 70 80 90 100 110 120 IHPFTGILMF FAFIYLALLY WDHNIPEKND IRWAKGVIEV LKGNEHAVAD NGKYNLGQKM 130 140 150 160 170 180 LFWTLNLAMV TLLVTGIIMW RQYFSHYFSI PVLRIAILLH SASAFMLFTG ILVHIYMAFW 190 200 210 220 230 VKGSIRGIVE GWVTVRWAKK HHPRWYREEV LSKLEEDLLN EQSGKVGKTK VLFKGFGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21686.1.
RefSeq	NP_438181.1.
3D structure databases
HSSP	HSSP built from PDB template 1KQF based on UniProtKB P24185.
ModBase	Search...
Genome annotation databases
GeneID	950906.
GenomeReviews	Gene locus HI0008 in contig L42023_GR.
KEGG	hin:HI0008.
NMPDR	fig|71421.1.peg.7.
TIGR	HI0008.
Phylogenomic databases
HOGENOM	P44451.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0008-MON.
Family and domain databases
InterPro	IPR006471. Formate_DHase_gsu. IPR000516. Ni-dep_hyd_CytB. [Graphical view]
PRINTS	PR00161. NIHGNASECYTB.
TIGRFAMs	TIGR01583. formate-DH-gamm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FDXI_HAEIN

Accession

Primary (citable) accession number: P44451

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)