Reviewed, UniProtKB/Swiss-Prot P44454 (LEP_HAEIN)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	HI0015

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Signal peptidase I		PRO_0000109507
Regions
	Transmembrane	3 – 23	21	Potential
	Transmembrane	25 – 45	21	Potential
	Topological domain	46 – 80	35	Cytoplasmic Potential
	Transmembrane	81 – 101	21	Potential
	Topological domain	102 – 349	248	Periplasmic Potential
Sites
	Active site	115	1	By similarity
	Active site	196	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44454-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8BAF091DCDF92F60	FASTA	349	39,734
	10 20 30 40 50 60 MSNLFFVILL AVGFGVWKVL DYFQLPNTFS ILLLILTALS GVLWCYHRFV VLPKRHRQVA 70 80 90 100 110 120 RAEQRSGKTL SEEEKAKIEP ISEASEFLSS LFPVLAVVFL VRSFLFEPFQ IPSGSMESTL 130 140 150 160 170 180 RVGDFLVVNK YAYGVKDPIF QNTIIAGEKP QRGDVIVFKA PQQALIRTGL GATRAAFAEN 190 200 210 220 230 240 LALSSKDNMS GVDYIKRIVG KGGDRVIFDV EQKTLKVVYG KEGKPCEIDC ETKAFEYTQN 250 260 270 280 290 300 PTNPAFPNEL ELTEKGDVTH NVLISEYRRY SDLEFFPQEG MQTAEWLVPE GQYFVMGDHR 310 320 330 340 DHSDDSRFWG FVPEKNIVGK ATYIWMSLEK EANEWPTGFR FERFFTAIK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21693.1.
PIR	H64042.
RefSeq	NP_438188.1.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Genome annotation databases
GeneID	950912.
GenomeReviews	Gene locus HI0015 in contig L42023_GR.
KEGG	hin:HI0015.
NMPDR	fig|71421.1.peg.14.
TIGR	HI0015.
Phylogenomic databases
HOGENOM	P44454.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0015-MON.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR011056. Peptidase_S24_S26_C. IPR014037. Peptidase_S26A. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
PANTHER	PTHR12383:SF1. Pept_S26A_signal_pept_1. 1 hit. PTHR12383. Peptidase_S26A. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_HAEIN

Accession

Primary (citable) accession number: P44454

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)