Reviewed, UniProtKB/Swiss-Prot P46616 (AA2AR_CAVPO)

Names and origin

Protein names

Recommended name:
    Adenosine receptor A2a

Gene names

Name:

ADORA2A

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 409	409	Adenosine receptor A2a
Regions
	Topological domain	1 – 4	4	Extracellular Potential
	Transmembrane	5 – 27	23	1 Potential
	Topological domain	28 – 40	13	Cytoplasmic Potential
	Transmembrane	41 – 63	23	2 Potential
	Topological domain	64 – 74	11	Extracellular Potential
	Transmembrane	75 – 97	23	3 Potential
	Topological domain	98 – 117	20	Cytoplasmic Potential
	Transmembrane	118 – 140	23	4 Potential
	Topological domain	141 – 170	30	Extracellular Potential
	Transmembrane	171 – 195	25	5 Potential
	Topological domain	196 – 231	36	Cytoplasmic Potential
	Transmembrane	232 – 255	24	6 Potential
	Topological domain	256 – 263	8	Extracellular Potential
	Transmembrane	264 – 287	24	7 Potential
	Topological domain	288 – 409	122	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Disulfide bond	74 ↔ 163		By similarity
Experimental info
	Sequence conflict	94	1	T → A Ref.2
	Sequence conflict	96	1	T → A Ref.2
	Sequence conflict	228	1	P → A in AAA18421. Ref.2
	Sequence conflict	243	1	C → W in AAA18421. Ref.2
	Sequence conflict	247	1	N → H in AAA18421. Ref.2
	Sequence conflict	260	1	D → G in AAA18421. Ref.2
	Sequence conflict	268 – 269	2	YL → SM in AAA18421. Ref.2
	Sequence conflict	349	1	L → P in AAA18421. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46616-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C4263E63B9000633	FASTA	409	44,943
	10 20 30 40 50 60 MSSSVYITVE LVIAVLAILG NVLVCWAVWI NSNLQNVTNY FVVSLAAADI AVGVLAIPFA 70 80 90 100 110 120 ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLTITIDRY IAIRIPLRYN GLVTCTRAKG 130 140 150 160 170 180 IIAICWVLSF AIGLTPMLGW NNCSQPKGDK NHSESCDEGQ VTCLFEDVVP MNYMVYYNFF 190 200 210 220 230 240 AFVLVPLLLM LGIYLRIFLA ARRQLKQMES QPLPGERTRS TLQKEVHPAK SLAIIVGLFA 250 260 270 280 290 300 LCCLPLNIIN CFTFFCPECD HAPPWLMYLT IILSHGNSVV NPLIYAYRIR EFRQTFRKII 310 320 330 340 350 360 RSHILRRREL FKAGGTSARA SAAHSPEGEQ VSLRLNGHPP GVWANGSALR PEQRPNGYVL 370 380 390 400 GLVSGRSAQR SHGDASLSDV ELLSHEHKGT CPESPSLEDP PAHGGAGVS

References

[1]	"Functional coupling of adenosine A2a receptor to inhibition of the mitogen-activated protein kinase cascade in Chinese hamster ovary cells." Hirano D., Ogasawara H., Kodama H., Waga I., Sakanaka C., Shimizu T., Nakamura M. Biochem. J. 316:81-86(1996) [PubMed: 8645236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Leukocyte.
[2]	"Cloning and expression of the A2a adenosine receptor from guinea pig brain." Meng F., Xie G.X., Chalmers D., Morgan C., Watson S.J., Akil H. Neurochem. Res. 19:613-621(1994) [PubMed: 8065518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Hartley. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D63674 mRNA. Translation: BAA09825.1. U04201 mRNA. Translation: AAA18421.1.
PIR	I48095. S68247.
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Phylogenomic databases
HOVERGEN	P46616.
Family and domain databases
InterPro	IPR001513. Adeno_A2A_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00553. ADENOSINA2AR. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P46616. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AA2AR_CAVPO

Accession

Primary (citable) accession number: P46616

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 2, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)