Reviewed, UniProtKB/Swiss-Prot P49578 (ACM3_CHICK)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M3

Gene names

Name:

CHRM3

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Tissue specificity	Brain, heart atria, and ventricle.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 639	639	Muscarinic acetylcholine receptor M3
Regions
	Topological domain	1 – 115	115	Extracellular Potential
	Transmembrane	116 – 139	24	1 Potential
	Topological domain	140 – 152	13	Cytoplasmic Potential
	Transmembrane	153 – 173	21	2 Potential
	Topological domain	174 – 190	17	Extracellular Potential
	Transmembrane	191 – 212	22	3 Potential
	Topological domain	213 – 232	20	Cytoplasmic Potential
	Transmembrane	233 – 255	23	4 Potential
	Topological domain	256 – 277	22	Extracellular Potential
	Transmembrane	278 – 300	23	5 Potential
	Topological domain	301 – 542	242	Cytoplasmic Potential
	Transmembrane	543 – 563	21	6 Potential
	Topological domain	564 – 576	13	Extracellular Potential
	Transmembrane	577 – 596	20	7 Potential
	Topological domain	597 – 639	43	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	16	1	N-linked (GlcNAc...) Potential
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	54	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Disulfide bond	189 ↔ 269		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P49578-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 7243F132B561B446	FASTA	639	71,977
	10 20 30 40 50 60 MLTHYQLCFQ KRSSQNYTVP DPTSCFDVPP WTILCQRATM IMHNNSSALP LFSNVSSFWK 70 80 90 100 110 120 RDSHGPGLLD EGASLIGSYD SPETTESFPF STVETTNSSL NATIKDPLGG HAVWQVVLIA 130 140 150 160 170 180 FLTGIIALVT IIGNILVIVS FKVNKQLKTV NNYFLLSLAC ADLIIGVISM NLFTTYIIMG 190 200 210 220 230 240 HWALGNLACD LWLSIDYVAS NASVMNLLVI SFDRYFSITR PLTYRAKRTT KRAGVMIGLA 250 260 270 280 290 300 WIISFVLWAP AILFWQYFVG KRTVPLDECF IQFLSEPIIT FGTAIAAFYL PVTIMSILYW 310 320 330 340 350 360 RIYKETEKRT KELAGLQASG SEAETARFVH QTGSSRSLSS YELQRQSTKR SSRRKYRRCH 370 380 390 400 410 420 FWLTMKSWEP NTDQGDQEHS SSDSWNNNDA AASLENSASS DEEDITAETR AIYSIVLKLP 430 440 450 460 470 480 GHSAILNSTK LPSSEDLNES ADELQKSDTD SQEKKPKKLQ PPKSIQDGGS FQKSFSKLPI 490 500 510 520 530 540 QPGSAETATA SDGISSVTKT SAALPLSFKE ATLAKKFALK TRSQITKRKR MSLIKEKKAA 550 560 570 580 590 600 QTLSAILFAF IITWTPYNIM VLVNTFCDCV PKTVWNLGYW LCYINSTVNP VCYALCNKMF 610 620 630 RNTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRIPREAS

References

[1]	"A novel M3 muscarinic acetylcholine receptor is expressed in chick atrium and ventricle." Gadbut A.P., Galper J.B. J. Biol. Chem. 269:25823-25829(1994) [PubMed: 7929287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10617 mRNA. Translation: AAA65961.1.
PIR	A55019.
RefSeq	NP_990730.1.
UniGene	Gga.781
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSGALG00000010778. Gallus gallus. [Contig view]
GeneID	396364.
KEGG	gga:396364.
Phylogenomic databases
HOGENOM	P49578.
HOVERGEN	P49578.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001183. Musac_M3_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF37. Musac_M3_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00540. MUSCRINICM3R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P49578. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM3_CHICK

Accession

Primary (citable) accession number: P49578

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)