Alanyl-tRNA synthetase, cytoplasmic

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS
    Renal carcinoma antigen NY-REN-42

Gene names

Name:

AARS

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subunit structure	Monomer.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Traceable author statement. Source: ProtInc tRNA processing Ref.1 Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc soluble fraction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	tRNA binding Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 968

968

Alanyl-tRNA synthetase, cytoplasmic

Regions

Region

1 – 497

497

Catalytic By similarity

Amino acid modifications

Modified residue

1

N-acetylmethionine

Modified residue

578

1

Phosphothreonine

Modified residue

580

1

Phosphotyrosine

Natural variations

Natural variant

275

1

G → D: dbSNP rs11537667.

Experimental info

Sequence conflict

82

1

H → Q in BAA06808. Ref.1

Sequence conflict

867

1

S → T in BAD96544. Ref.2

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Sequences

Sequence

Length

Mass (Da)

Tools

P49588-1 [UniParc].

Last modified October 3, 2006. Version 2.
Checksum: 8683F111CEE42506
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FASTA

968

106,810

        10         20         30         40         50         60 
MDSTLTASEI RQRFIDFFKR NEHTYVHSSA TIPLDDPTLL FANAGMNQFK PIFLNTIDPS 

        70         80         90        100        110        120 
HPMAKLSRAA NTQKCIRAGG KHNDLDDVGK DVYHHTFFEM LGSWSFGDYF KELACKMALE 

       130        140        150        160        170        180 
LLTQEFGIPI ERLYVTYFGG DEAAGLEADL ECKQIWQNLG LDDTKILPGN MKDNFWEMGD 

       190        200        210        220        230        240 
TGPCGPCSEI HYDRIGGRDA AHLVNQDDPN VLEIWNLVFI QYNREADGIL KPLPKKSIDT 

       250        260        270        280        290        300 
GMGLERLVSV LQNKMSNYDT DLFVPYFEAI QKGTGARPYT GKVGAEDADG IDMAYRVLAD 

       310        320        330        340        350        360 
HARTITVALA DGGRPDNTGR GYVLRRILRR AVRYAHEKLN ASRGFFATLV DVVVQSLGDA 

       370        380        390        400        410        420 
FPELKKDPDM VKDIINEEEV QFLKTLSRGR RILDRKIQSL GDSKTIPGDT AWLLYDTYGF 

       430        440        450        460        470        480 
PVDLTGLIAE EKGLVVDMDG FEEERKLAQL KSQGKGAGGE DLIMLDIYAI EELRARGLEV 

       490        500        510        520        530        540 
TDDSPKYNYH LDSSGSYVFE NTVATVMALR REKMFVEEVS TGQECGVVLD KTCFYAEQGG 

       550        560        570        580        590        600 
QIYDEGYLVK VDDSSEDKTE FTVKNAQVRG GYVLHIGTIY GDLKVGDQVW LFIDEPRRRP 

       610        620        630        640        650        660 
IMSNHTATHI LNFALRSVLG EADQKGSLVA PDRLRFDFTA KGAMSTQQIK KAEEIANEMI 

       670        680        690        700        710        720 
EAAKAVYTQD CPLAAAKAIQ GLRAVFDETY PDPVRVVSIG VPVSELLDDP SGPAGSLTSV 

       730        740        750        760        770        780 
EFCGGTHLRN SSHAGAFVIV TEEAIAKGIR RIVAVTGAEA QKALRKAESL KKCLSVMEAK 

       790        800        810        820        830        840 
VKAQTAPNKD VQREIADLGE ALATAVIPQW QKDELRETLK SLKKVMDDLD RASKADVQKR 

       850        860        870        880        890        900 
VLEKTKQFID SNPNQPLVIL EMESGASAKA LNEALKLFKM HSPQTSAMLF TVDNEAGKIT 

       910        920        930        940        950        960 
CLCQVPQNAA NRGLKASEWV QQVSGLMDGK GGGKDVSAQA TGKNVGCLQE ALQLATSFAQ 


LRLGDVKN

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition." Shiba K., Ripmaster T.L., Suzuki N., Nichols R., Plotz P., Noda T., Schimmel P. Biochemistry 34:10340-10349(1995) [PubMed: 7654687] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	Bienvenut W.V., Glen H., Brunton V.G., Frame M.C. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11; 304-320 AND 684-695, ACETYLATION AT MET-1, MASS SPECTROMETRY. Tissue: Osteosarcoma.
[7]	"Antigens recognized by autologous antibody in patients with renal-cell carcinoma." Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J. Int. J. Cancer 83:456-464(1999) [PubMed: 10508479] [Abstract] Cited for: IDENTIFICATION AS A RENAL CANCER ANTIGEN. Tissue: Renal cell carcinoma.
[8]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-578 AND TYR-580, MASS SPECTROMETRY. Tissue: Epithelium.

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Cross-references

Sequence databases
	D32050 mRNA. Translation: BAA06808.1. AK222824 mRNA. Translation: BAD96544.1. AC012184 Genomic DNA. No translation available. CH471241 Genomic DNA. Translation: EAW51839.1. BC011451 mRNA. Translation: AAH11451.1.
PIR	I60107.
RefSeq	NP_001596.2.
UniGene	Hs.315137
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P49588.
PTM databases
PhosphoSite	P49588.
Genome annotation databases
Ensembl	ENSG00000090861. Homo sapiens. [Contig view]
GeneID	16.
KEGG	hsa:16.
Organism-specific databases
H-InvDB	HIX0021867.
HGNC	HGNC:20. AARS.
MIM	601065. gene.
PharmGKB	PA24367.
GenAtlas	Search...
GeneCards	Search...
GeneLynx	Search...
Phylogenomic databases
HOVERGEN	P49588.
Gene expression databases
ArrayExpress	P49588.
CleanEx	HS_AARS.
GermOnline	ENSG00000090861. Homo sapiens.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	P49588. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
DrugBank	DB00160. L-Alanine.
SOURCE	Search...
ProtoNet	Search...

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Entry information

Entry name

SYAC_HUMAN

Accession

Primary (citable) accession number: P49588
Secondary accession number(s): A6NF14, Q53GV7, Q96FA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	October 3, 2006
Last modified:	July 22, 2008
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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