Reviewed, UniProtKB/Swiss-Prot P50978 (LACG_STRMU)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SMU_1490

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol.
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity
Experimental info
	Sequence conflict	150	1	P → S in AAA16450. Ref.1
	Sequence conflict	300	1	M → I in AAA16450. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P50978-1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: FEDBDCE1E23D84F7	FASTA	468	53,762
	10 20 30 40 50 60 MSKTLPKDFI FGGATAAYQA EGATHADGKG PVAWDKYLED NYWYTAEPAS DFYHQYPVDL 70 80 90 100 110 120 KLAEEFGVNG IRISIAWSRI FPKGYGAVNP KGLAFYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSNGDFL NRENIEHFVN YAEFCFKEFP EVNYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVAHSKAV KLFKDGGYSG EIGVVHALPT KYPYDPNNPA DIRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGKYSEKTM EGVNHILAVN GGQLDLREED FAALEAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMRAFDGE TEITHNAKGE KGSSKYQIKG VGRREAPVNV PKTDWDWIIY PQGLYDQIMR 370 380 390 400 410 420 VKQDYPNYKK IYITENGLGY KDEFVNHTVY DDARIDYVKK HLEVLSDAIA DGANVKGYFI 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFDTQERY PKKSAYWYKK LAETQIID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene." Honeyman A.L., Curtiss R. III J. Gen. Microbiol. 139:2685-2694(1993) [PubMed: 8277252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 700611 / UA130 / Serotype c.
[2]	"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18993 Unassigned DNA. Translation: AAA16450.1. AE014133 Genomic DNA. Translation: AAN59144.1.
RefSeq	NP_721838.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	P50978. Positions 1-468.
ModBase	Search...
Genome annotation databases
GeneID	1028734.
GenomeReviews	Gene locus SMU_1490 in contig AE014133_GR.
KEGG	smu:SMU.1490.
NMPDR	fig|210007.1.peg.1351.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P50978.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_1490-MON.
Family and domain databases
HAMAP	MF_01574. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_sub_cat. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProDom	P50978. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

LACG_STRMU

Accession

Primary (citable) accession number: P50978

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 28, 2002
Last modified:	September 2, 2008
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)