Reviewed, UniProtKB/Swiss-Prot P51996 (YPT32_YEAST)

Names and origin

Protein names

Recommended name:
    GTP-binding protein YPT32/YPT11
Alternative name(s):
    Rab GTPase YPT32

Gene names

Name:	YPT32
Synonyms:	YPT11
Ordered Locus Names:	YGL210W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for protein transport through the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Mediates the proper polarized localization of SEC2, a GEF for SEC4, but does not alter the exchange activity of SEC2 on SEC4.
Enzyme regulation	Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.
Subunit structure	Interacts with YIF1, YIP3, YIP4 and YIP5. Interacts with the GEF SEC2.
Subcellular location	Golgi apparatus membrane; Lipid-anchor.
Miscellaneous	Present with 4298 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Exocytosis Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	exocytosis Ref.6 Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD mitochondrial outer membrane Inferred from direct assay. Source: SGD
Molecular function	GTPase activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 222	221	GTP-binding protein YPT32/YPT11
Regions
	Nucleotide binding	20 – 27	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	126 – 129	4	GTP By similarity
	Motif	42 – 50	9	Effector region Probable
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Lipidation	221	1	S-geranylgeranyl cysteine By similarity
	Lipidation	222	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Mutagenesis	27	1	S → N: Binds preferentially GDP
	Mutagenesis	49	1	E → Q: Cold sensitive
	Mutagenesis	72	1	Q → L: Reduces GTPase activity
	Mutagenesis	126	1	N → I: Blocks nucleotide binding
	Mutagenesis	141	1	A → D: Loss of function at 37 degrees Celsius

Sequences

	Sequence		Length	Mass (Da)
	P51996-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 621217A296161964	FASTA	222	24,520
	10 20 30 40 50 60 MSNEDYGYDY DYLFKIVLIG DSGVGKSNLL SRFTTDEFNI ESKSTIGVEF ATRTIEVENK 70 80 90 100 110 120 KIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLTE LRENADDNVA 130 140 150 160 170 180 VGLIGNKSDL AHLRAVPTDE AKNFAMENQM LFTETSALNS DNVDKAFREL IVAIFQMVSK 190 200 210 220 HQVDLSGSGT NNMGSNGAPK GPTISLTPAP KEDKKKKSSN CC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast." Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D. EMBO J. 15:6460-6475(1996) [PubMed: 8978673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: DUR.
[2]	"Lambda clone B22 contains a 7676 bp genomic fragment of Saccharomyces cerevisiae chromosome VII spanning the VAM7-SPM2 intergenic region and containing three novel transcribed open reading frames." Kail M., Juettner E., Vaux D. Yeast 12:799-807(1996) [PubMed: 8813766] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Analysis of 21.7 kb DNA sequence from the left arm of chromosome VII reveals 11 open reading frames: two correspond to new genes." Feuermann M., Simeonava L., Souciet J.-L., Potier S. Yeast 13:475-477(1997) [PubMed: 9153757] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment." Jedd G., Mulholland J., Segev N. J. Cell Biol. 137:563-580(1997) [PubMed: 9151665] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ALA-141, SUBCELLULAR LOCATION.
[7]	"Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition." Albert S., Gallwitz D. J. Biol. Chem. 274:33186-33189(1999) [PubMed: 10559187] [Abstract] Cited for: ENZYME REGULATION.
[8]	"The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32." Jones S., Newman C., Liu F., Segev N. Mol. Biol. Cell 11:4403-4411(2000) [PubMed: 11102533] [Abstract] Cited for: ENZYME REGULATION.
[9]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed: 11785952] [Abstract] Cited for: INTERACTION WITH YIP3.
[10]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed: 11943201] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[11]	"Ypt32 recruits the Sec4p guanine nucleotide exchange factor, Sec2p, to secretory vesicles; evidence for a Rab cascade in yeast." Ortiz D., Medkova M., Walch-Solimena C., Novick P.J. J. Cell Biol. 157:1005-1015(2002) [PubMed: 12045183] [Abstract] Cited for: FUNCTION, INTERACTION WITH SEC2, MUTAGENESIS OF SER-27; GLU-49; GLN-72 AND ASN-126.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72834 Genomic DNA. Translation: CAA51355.1. U33754 Genomic DNA. Translation: AAC49495.1. Z72732 Genomic DNA. Translation: CAA96926.1. AY558506 Genomic DNA. Translation: AAS56832.1.
PIR	S58514.
RefSeq	NP_011305.1.
3D structure databases
HSSP	HSSP built from PDB template 1G16 based on UniProtKB P07560.
SMR	P51996. Positions 9-182, 10-183.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6597N.
IntAct	P51996.
Proteomic databases
PeptideAtlas	P51996.
Genome annotation databases
Ensembl	YGL210W. Saccharomyces cerevisiae. [Contig view]
GeneID	852662.
GenomeReviews	Gene locus YGL210W in contig Y13135_GR.
KEGG	sce:YGL210W.
NMPDR	fig|4932.3.peg.2408.
Organism-specific databases
CYGD	YGL210w.
SGD	S000003178. YPT32.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P51996.
Gene expression databases
GermOnline	YGL210W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003579. GTPase_Rab. IPR015595. Rab11. IPR013753. Ras. IPR001806. Ras_trnsfrmng. IPR005225. Small_GTP_bd. [Graphical view]
PANTHER	PTHR11708:SF213. Rab11. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00175. RAB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
ProDom	P51996. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P51996.
ProtoNet	Search...

Entry information

Entry name

YPT32_YEAST

Accession

Primary (citable) accession number: P51996

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	July 22, 2008
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)