Reviewed, UniProtKB/Swiss-Prot P52722 (MT1_DANRE)

Names and origin

Protein names

Recommended name:
    Metallothionein-1
      Short name(s)=MT-1

Gene names

Name:	mt
Synonyms:	mt1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	60 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals By similarity.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
Gene Ontology (GO)
Biological process	response to cadmium ion Inferred from direct assay. Source: ZFIN
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 60	60	Metallothionein-1
Regions
	Region	1 – 28	28	Beta
	Region	29 – 60	32	Alpha
Sites
	Metal binding	4	1	Cluster B
	Metal binding	6	1	Cluster B
	Metal binding	12	1	Cluster B
	Metal binding	14	1	Cluster B
	Metal binding	18	1	Cluster B
	Metal binding	20	1	Cluster B
	Metal binding	23	1	Cluster B
	Metal binding	25	1	Cluster B
	Metal binding	28	1	Cluster B
	Metal binding	32	1	Cluster A
	Metal binding	33	1	Cluster A
	Metal binding	35	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	40	1	Cluster A
	Metal binding	43	1	Cluster A
	Metal binding	47	1	Cluster A
	Metal binding	49	1	Cluster A
	Metal binding	54	1	Cluster A
	Metal binding	58	1	Cluster A
	Metal binding	59	1	Cluster A

Sequences

	Sequence		Length	Mass (Da)
	P52722-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: E5223E7C292BB700	FASTA	60	5,960
	10 20 30 40 50 60 MDPCECAKTG ACNCGATCKC TNCQCTTCKK SCCSCCPSGC SKCASGCVCK GNSCGTSCCQ

References

[1]	"The use of metallothionein genes for determining the phylogenetic and evolutionary relationship between extant teleosts." Kille P., Olsson P.-E. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97278 mRNA. Translation: CAA65933.1.
RefSeq	NP_571150.1.
UniGene	Dr.20068
3D structure databases
HSSP	HSSP built from PDB template 1M0G based on UniProtKB O73914.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDARG00000041623. Danio rerio. [Contig view]
GeneID	30282.
KEGG	dre:30282.
Organism-specific databases
ZFIN	ZDB-GENE-990415-159. mt.
Phylogenomic databases
HOGENOM	P52722.
HOVERGEN	P52722.
Gene expression databases
ArrayExpress	P52722.
Family and domain databases
InterPro	IPR015874. 4-disulphide_core. IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00003. 4DISULPHCORE. PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P52722. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MT1_DANRE

Accession

Primary (citable) accession number: P52722

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project