tRNA-specific adenosine deaminase 1 - Saccharomyces cerevisiae (Baker's yeast)

Contribute

Reviewed, UniProtKB/Swiss-Prot P53065 (TAD1_YEAST)

Last modified July 22, 2008. Version 52. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    tRNA-specific adenosine deaminase 1
    EC=3.5.4.-
Alternative name(s):
    tRNA-specific adenosine-37 deaminase

Gene names

Name:	TAD1
Ordered Locus Names:	YGL243W
ORF Names:	HRA400

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function

Deaminates adenosine-37 to inosine in tRNA-Ala.

Cofactor

Binds 1 inositol hexakisphosphate (IP6) per subunit.

Zinc By similarity.

Sequence similarities

Belongs to the ADAT1 family.

Contains 1 A to I editase domain.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Ref.1 Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	tRNA-specific adenosine-37 deaminase activity Ref.1 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

400

tRNA-specific adenosine deaminase 1

Regions

Domain

325

A to I editase

Sites

Active site

1

Proton donor By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Metal binding

1

Zinc By similarity

Binding site

1

Inositol hexakisphosphate By similarity

Binding site

1

Inositol hexakisphosphate By similarity

Binding site

1

Inositol hexakisphosphate By similarity

Binding site

1

Inositol hexakisphosphate By similarity

Binding site

1

Inositol hexakisphosphate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P53065-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2AA9304C549026FF
Show »

400

45,947

        10         20         30         40         50         60 
MVSCQGTRPC IVNLLTMPSE DKLGEEISTR VINEYSKLKS ACRPIIRPSG IREWTILAGV 

        70         80         90        100        110        120 
AAINRDGGAN KIEILSIATG VKALPDSELQ RSEGKILHDC HAEILALRGA NTVLLNRIQN 

       130        140        150        160        170        180 
YNPSSGDKFI QHNDEIPARF NLKENWELAL YISRLPCGDA SMSFLNDNCK NDDFIKIEDS 

       190        200        210        220        230        240 
DEFQYVDRSV KTILRGRLNF NRRNVVRTKP GRYDSNITLS KSCSDKLLMK QRSSVLNCLN 

       250        260        270        280        290        300 
YELFEKPVFL KYIVIPNLED ETKHHLEQSF HTRLPNLDNE IKFLNCLKPF YDDKLDEEDV 

       310        320        330        340        350        360 
PGLMCSVKLF MDDFSTEEAI LNGVRNGFYT KSSKPLRKHC QSQVSRFAQW ELFKKIRPEY 

       370        380        390        400 
EGISYLEFKS RQKKRSQLII AIKNILSPDG WIPTRTDDVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2." Gerber A., Grosjean H., Melcher T., Keller W. EMBO J. 17:4780-4789(1998) [PubMed: 9707437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: BMA41.
[2]	"The sequence of an 11.1 kb DNA fragment between ADH4 and ADE5 on the left arm of chromosome VII, reveals the presence of eight open reading frames." Vandenbol M., Durand P., Portetelle D., Hilger F. Yeast 11:1519-1523(1995) [PubMed: 8750240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing." Macbeth M.R., Schubert H.L., Vandemark A.P., Lingam A.T., Hill C.P., Bass B.L. Science 309:1534-1539(2005) [PubMed: 16141067] [Abstract] Cited for: COFACTOR.

Cross-references

Sequence databases
	AJ007297 Genomic DNA. Translation: CAA07438.1. Z49149 Genomic DNA. Translation: CAA89012.1. Z72765 Genomic DNA. Translation: CAA96962.1.
PIR	S53937.
RefSeq	NP_011271.1.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	YGL243W. Saccharomyces cerevisiae. [Contig view]
GeneID	852608.
GenomeReviews	Gene locus YGL243W in contig Y13135_GR.
KEGG	sce:YGL243W.
NMPDR	fig\|4932.3.peg.2374.
Organism-specific databases
CYGD	YGL243w.
SGD	S000003212. TAD1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53065.
Gene expression databases
GermOnline	YGL243W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002466. A_deamin. [Graphical view]
Pfam	PF02137. A_deamin. 1 hit. [Graphical view]
SMART	SM00552. ADEAMc. 1 hit. [Graphical view]
PROSITE	PS50141. A_DEAMIN_EDITASE. 1 hit. [Graphical view]
ProDom	P53065. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P53065.
ProtoNet	Search...

Entry information

Entry name

TAD1_YEAST

Accession

Primary (citable) accession number: P53065

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents