Reviewed, UniProtKB/Swiss-Prot P53072 (TAN1_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA acetyltransferase TAN1
    EC=2.3.-.-

Gene names

Name:	TAN1
Ordered Locus Names:	YGL232W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable tRNA acetyltransferase required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs. Binds RNA.
Subcellular location	Cytoplasm. Nucleus.
Miscellaneous	Present with 2630 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	RNA binding Ref.2 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 289	289	tRNA acetyltransferase TAN1
Regions
	Domain	146 – 259	114	THUMP
Amino acid modifications
	Modified residue	72	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P53072-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0E1C8C55E70CEB28	FASTA	289	33,550
	10 20 30 40 50 60 MGEKRNRNGK DANSQNRKKF KVSSGFLDPG TSGIYATCSR RHERQAAQEL QLLFEEKFQE 70 80 90 100 110 120 LYGDIKEGED ESENDEKKDL SIEDQIKKEL QELKGEETGK DLSSGETKKK DPLAFIDLNC 130 140 150 160 170 180 ECVTFCKTRK PIVPEEFVLS IMKDLADPKN MVKRTRYVQK LTPITYSCNA KMEQLIKLAN 190 200 210 220 230 240 LVIGPHFHDP SNVKKNYKFA VEVTRRNFNT IERMDIINQV VKLVNKEGSE FNHTVDLKNY 250 260 270 280 DKLILVECFK SNIGMCVVDG DYKTKYRKYN VQQLYESKFR KDEDKSVKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The Saccharomyces cerevisiae TAN1 gene is required for N4-acetylcytidine formation in tRNA." Johansson M.J., Bystrom A.S. RNA 10:712-719(2004) [PubMed: 15037780] [Abstract] Cited for: FUNCTION.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72, MASS SPECTROMETRY.
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z72754 Genomic DNA. Translation: CAA96950.1.
PIR	S64254.
RefSeq	NP_011282.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P53072.
Proteomic databases
PeptideAtlas	P53072.
Genome annotation databases
Ensembl	YGL232W. Saccharomyces cerevisiae. [Contig view]
GeneID	852619.
GenomeReviews	Gene locus YGL232W in contig Y13135_GR.
KEGG	sce:YGL232W.
NMPDR	fig|4932.3.peg.2385.
Organism-specific databases
CYGD	YGL232w.
SGD	S000003201. TAN1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53072.
Gene expression databases
GermOnline	YGL232W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004114. THUMP. [Graphical view]
Pfam	PF02926. THUMP. 1 hit. [Graphical view]
PROSITE	PS51165. THUMP. 1 hit. [Graphical view]
ProDom	P53072. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P53072.
ProtoNet	Search...

Entry information

Entry name

TAN1_YEAST

Accession

Primary (citable) accession number: P53072

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 2, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)