Reviewed, UniProtKB/Swiss-Prot P53091 (MCM6_YEAST)

Names and origin

Protein names

Recommended name:
    DNA replication licensing factor MCM6
Alternative name(s):
    Minichromosome maintenance protein 6

Gene names

Name:	MCM6
Ordered Locus Names:	YGL201C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1017 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with MCM10.
Subcellular location	NucleusPotential.
Miscellaneous	Present with 13400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the MCM family. Contains 1 MCM domain.

Ontologies

Keywords
Biological process	Cell cycle DNA replication Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication initiation Inferred from physical interaction. Source: SGD DNA strand elongation during DNA replication Inferred from mutant phenotype. Source: SGD DNA unwinding during replication Inferred from direct assay. Source: SGD S phase of mitotic cell cycle Inferred from mutant phenotype. Source: SGD pre-replicative complex assembly Inferred from direct assay. Source: SGD
Cellular component	DNA replication preinitiation complex Inferred from direct assay. Source: SGD MCM complex Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay. Source: SGD pre-replicative complex Inferred from direct assay. Source: SGD
Molecular function	DNA replication origin binding Inferred from direct assay. Source: SGD four-way junction helicase activity Inferred from direct assay. Source: SGD protein binding Ref.4 Inferred from physical interaction. Source: IntAct single-stranded DNA-dependent ATPase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MCM10	P32354	3	EBI-10556,EBI-5965

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1017	1017	DNA replication licensing factor MCM6
Regions
	Domain	525 – 732	208	MCM
	Nucleotide binding	575 – 582	8	ATP Potential
Amino acid modifications
	Modified residue	31	1	Phosphoserine
	Modified residue	78	1	Phosphoserine
	Modified residue	249	1	Phosphoserine
	Modified residue	372	1	Phosphoserine
	Modified residue	766	1	Phosphothreonine
	Modified residue	1016	1	Phosphoserine
	Modified residue	1017	1	Phosphoserine
Experimental info
	Sequence conflict	179	1	A → P in AAO89010. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53091-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 6DA86379ABBDF774	FASTA	1,017	112,952
	10 20 30 40 50 60 MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP 70 80 90 100 110 120 FVNDSTQFSS QRLQTDGSAT NDMEGNEPAR SFKSRALNHV KKVDDVTGEK VREAFEQFLE 130 140 150 160 170 180 DFSVQSTDTG EVEKVYRAQI EFMKIYDLNT IYIDYQHLSM RENGALAMAI SEQYYRFLAF 190 200 210 220 230 240 LQKGLRRVVR KYAPELLNTS DSLKRSEGDE GQADEDEQQD DDMNGSSLPR DSGSSAAPGN 250 260 270 280 290 300 GTSAMATRSI TTSTSPEQTE RVFQISFFNL PTVHRIRDIR SEKIGSLLSI SGTVTRTSEV 310 320 330 340 350 360 RPELYKASFT CDMCRAIVDN VEQSFKYTEP TFCPNPSCEN RAFWTLNVTR SRFLDWQKVR 370 380 390 400 410 420 IQENANEIPT GSMPRTLDVI LRGDSVERAK PGDRCKFTGV EIVVPDVTQL GLPGVKPSST 430 440 450 460 470 480 LDTRGISKTT EGLNSGVTGL RSLGVRDLTY KISFLACHVI SIGSNIGASS PDANSNNRET 490 500 510 520 530 540 ELQMAANLQA NNVYQDNERD QEVFLNSLSS DEINELKEMV KDEHIYDKLV RSIAPAVFGH 550 560 570 580 590 600 EAVKKGILLQ MLGGVHKSTV EGIKLRGDIN ICVVGDPSTS KSQFLKYVVG FAPRSVYTSG 610 620 630 640 650 660 KASSAAGLTA AVVRDEEGGD YTIEAGALML ADNGICCIDE FDKMDISDQV AIHEAMEQQT 670 680 690 700 710 720 ISIAKAGIHA TLNARTSILA AANPVGGRYN RKLSLRGNLN MTAPIMSRFD LFFVILDDCN 730 740 750 760 770 780 EKIDTELASH IVDLHMKRDE AIEPPFSAEQ LRRYIKYART FKPILTKEAR SYLVEKYKEL 790 800 810 820 830 840 RKDDAQGFSR SSYRITVRQL ESMIRLSEAI ARANCVDEIT PSFIAEAYDL LRQSIIRVDV 850 860 870 880 890 900 DDVEMDEEFD NIESQSHAAS GNNDDNDDGT GSGVITSEPP ADIEEGQSEA TARPGTSEKK 910 920 930 940 950 960 KTTVTYDKYV SMMNMIVRKI AEVDREGAEE LTAVDIVDWY LLQKENDLGS LAEYWEERRL 970 980 990 1000 1010 AFKVIKRLVK DRILMEIHGT RHNLRDLENE ENENNKTVYV IHPNCEVLDQ LEPQDSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the Saccharomyces cerevisiae MCM6 gene." Duina A.A., Winston F. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Characterisation of Saccharomyces cerevisiae ARO8 and ARO9 genes encoding aromatic aminotransferases I and II reveals a new aminotransferase subfamily." Iraqui I., Vissers S., Cartiaux M., Urrestarazu A. Mol. Gen. Genet. 257:238-248(1998) [PubMed: 9491083] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 456-1017. Strain: Sigma 1278B.
[4]	"A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae." Merchant A.M., Kawasaki Y., Chen Y., Lei M., Tye B.K. Mol. Cell. Biol. 17:3261-3271(1997) [PubMed: 9154825] [Abstract] Cited for: INTERACTION WITH MCM10.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-766, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 0:0-0(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-78; SER-249; SER-372; SER-1016 AND SER-1017, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY258324 Genomic DNA. Translation: AAO89010.1. Z72723 Genomic DNA. Translation: CAA96913.1. Y13624 Genomic DNA. Translation: CAA73947.1.
PIR	S64219.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1294N.
IntAct	P53091.
Proteomic databases
PeptideAtlas	P53091.
Genome annotation databases
Ensembl	YGL201C. Saccharomyces cerevisiae. [Contig view]
GenomeReviews	Gene locus YGL201C in contig Y13135_GR.
KEGG	sce:YGL201C.
Organism-specific databases
CYGD	YGL201c.
SGD	S000003169. MCM6.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53091.
Gene expression databases
ArrayExpress	P53091.
GermOnline	YGL201C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001208. MCM. IPR008049. MCM_6. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR11630. MCM. 1 hit.
Pfam	PF00493. MCM. 1 hit. [Graphical view]
PRINTS	PR01657. MCMFAMILY. PR01662. MCMPROTEIN6.
ProDom	PD001041. MCM. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00350. MCM. 1 hit. [Graphical view]
PROSITE	PS00847. MCM_1. 1 hit. PS50051. MCM_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
LinkHub	P53091.
ProtoNet	Search...

Entry information

Entry name

MCM6_YEAST

Accession

Primary (citable) accession number: P53091
Secondary accession number(s): Q870M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)