Reviewed, UniProtKB/Swiss-Prot P53101 (STR3_YEAST)

Names and origin

Protein names

Recommended name:
    Cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Name:	STR3
Ordered Locus Names:	YGL184C
ORF Names:	G1601

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subcellular location	Cytoplasm. Nucleus.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Ref.3 Inferred from mutant phenotype. Source: SGD
Molecular function	cystathionine beta-lyase activity Ref.3 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRP40	P33203	1	EBI-24097,EBI-701
RSP5	P39940	1	EBI-24097,EBI-16219
SSM4	P40318	1	EBI-24097,EBI-18208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 465	465	Cystathionine beta-lyase
Sites
	Binding site	213	1	Pyridoxal phosphate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53101-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 50957D6A2D7EDB43	FASTA	465	51,828
	10 20 30 40 50 60 MPIKRLDTVV VNTGSQNDQH SASVPPVYLS TTFKVDLNNE DAQNYDYSRS GNPTRSVLQH 70 80 90 100 110 120 QIGKLYRVPQ ENVLAVSSGM TALDVILRGL VLLNGTDNHT PTIIAGDDLY GGTQRLLNFF 130 140 150 160 170 180 KQQSHAVSVH VDTSDFEKFK TVFQSLDKVD CVLLESPTNP LCKVVDIPRI LRFVKCISPD 190 200 210 220 230 240 TTVVVDNTMM SGLNCNPLQL NPGCDVVYES ATKYLNGHHD LMGGVIISKT PEIASKLYFV 250 260 270 280 290 300 INSTGAGLSP MDSWLLVRGL KTLGVRLYQQ QRNAMILAHW LENSCGFKPT RTNKATKTRF 310 320 330 340 350 360 VGLRSNPDFK LHKSFNNGPG AVLSFETGSF EHSKRLVSSK KLSIWAVTVS FGCVNSLLSM 370 380 390 400 410 420 PCKMSHASID PELRKERDFP EDLVRLCCGI ENIVDLKKDL LAAMVDADII EVRENGKYLF 430 440 450 460 NKLNKNLAVN TTIDDLHKPL SIYEEFYNQD LIRKDSELNI KSSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII from Saccharomyces cerevisiae." Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V. Yeast 13:55-64(1997) [PubMed: 9046087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae." Hansen J., Johannesen P.F. Mol. Gen. Genet. 263:535-542(2000) [PubMed: 10821189] [Abstract] Cited for: IDENTIFICATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91489 Genomic DNA. Translation: CAA62790.1. Z72706 Genomic DNA. Translation: CAA96896.1.
PIR	S61133.
RefSeq	NP_011331.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBJ based on UniProtKB P53780.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4518N.
IntAct	P53101.
Genome annotation databases
Ensembl	YGL184C. Saccharomyces cerevisiae. [Contig view]
GeneID	852691.
GenomeReviews	Gene locus YGL184C in contig Y13135_GR.
KEGG	sce:YGL184C.
NMPDR	fig|4932.3.peg.2435.
Organism-specific databases
CYGD	YGL184c.
SGD	S000003152. STR3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53101.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-391.
Gene expression databases
GermOnline	YGL184C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006238. Cys_b_lyase_euk. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01329. cysta_beta_ly_E. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProDom	P53101. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P53101.
ProtoNet	Search...

Entry information

Entry name

STR3_YEAST

Accession

Primary (citable) accession number: P53101

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 2, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)