Reviewed, UniProtKB/Swiss-Prot P53367 (ARFP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Arfaptin-1
Alternative name(s):
    ADP-ribosylation factor-interacting protein 1

Gene names

Name:

ARFIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative target protein of ADP-ribosylation factor.
Subunit structure	Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.
Tissue specificity	Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.
Sequence similarities	Contains 1 AH domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Gene Ontology (GO)
Cellular component	Golgi membrane Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P53367-1)

Also known as: 1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P53367-2)

Also known as: 1a;

The sequence of this isoform differs from the canonical sequence as follows:
     69-100: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 373	373	Arfaptin-1
Regions
	Domain	153 – 353	201	AH
Natural variations
	Alternative sequence	69 – 100	32	Missing in isoform A.

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (1b) [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 7A315D0E67DECDBF	FASTA	373	41,738
	10 20 30 40 50 60 MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV 70 80 90 100 110 120 IEAGAFQGSP APPLPSVMSP SRVAASRLAQ QGSDLIVPAG GQRTQTKSGP VILADEIKNP 130 140 150 160 170 180 AMEKLELVRK WSLNTYKCTR QIISEKLGRG SRTVDLELEA QIDILRDNKK KYENILKLAQ 190 200 210 220 230 240 TLSTQLFQMV HTQRQLGDAF ADLSLKSLEL HEEFGYNADT QKLLAKNGET LLGAINFFIA 250 260 270 280 290 300 SVNTLVNKTI EDTLMTVKQY ESARIEYDAY RTDLEELNLG PRDANTLPKI EQSQHLFQAH 310 320 330 340 350 360 KEKYDKMRND VSVKLKFLEE NKVKVLHNQL VLFHNAIAAY FAGNQKQLEQ TLKQFHIKLK 370 TPGVDAPSWL EEQ
	Isoform A (1a) [UniParc]. Checksum: 94675E3FF3FB9D08		341	38,599
	10 20 30 40 50 60 MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV 70 80 90 100 110 120 IEAGAFQGGQ RTQTKSGPVI LADEIKNPAM EKLELVRKWS LNTYKCTRQI ISEKLGRGSR 130 140 150 160 170 180 TVDLELEAQI DILRDNKKKY ENILKLAQTL STQLFQMVHT QRQLGDAFAD LSLKSLELHE 190 200 210 220 230 240 EFGYNADTQK LLAKNGETLL GAINFFIASV NTLVNKTIED TLMTVKQYES ARIEYDAYRT 250 260 270 280 290 300 DLEELNLGPR DANTLPKIEQ SQHLFQAHKE KYDKMRNDVS VKLKFLEENK VKVLHNQLVL 310 320 330 340 FHNAIAAYFA GNQKQLEQTL KQFHIKLKTP GVDAPSWLEE Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes." Kanoh H., Williger B.-T., Exton J.H. J. Biol. Chem. 272:5421-5429(1997) [PubMed: 9038142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"Arfaptin-1b, a long splice variant of arfaptin-1." Premont R.T., Lefkowitz R.J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Tissue: Uterus.
[4]	"Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D." Williger B.-T., Provost J.J., Ho W.-T., Milstine J., Exton J.H. FEBS Lett. 454:85-89(1999) [PubMed: 10413101] [Abstract] Cited for: INTERACTION WITH ARF1; ARF3; ARF5 AND ARF6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52521 mRNA. Translation: AAA97923.1. AF124489 mRNA. Translation: AAD29390.1. BC103759 mRNA. Translation: AAI03760.1.
PIR	G02515.
RefSeq	NP_001020764.1. NP_001020766.1. NP_055262.1.
UniGene	Hs.416089
3D structure databases
HSSP	HSSP built from PDB template 1I4L based on UniProtKB P53365.
SMR	P53367. Positions 151-351.
ModBase	Search...
PTM databases
PhosphoSite	P53367.
Genome annotation databases
Ensembl	ENSG00000164144. Homo sapiens. [Contig view]
GeneID	27236.
KEGG	hsa:27236.
Organism-specific databases
H-InvDB	HIX0004575.
HGNC	HGNC:21496. ARFIP1.
HPA	CAB005080.
MIM	605928. gene.
PharmGKB	PA134920621.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P53367.
HOVERGEN	P53367.
Gene expression databases
ArrayExpress	P53367.
CleanEx	HS_ARFIP1.
GermOnline	ENSG00000164144. Homo sapiens.
Family and domain databases
InterPro	IPR010504. Arfaptin. [Graphical view]
Gene3D	G3DSA:1.20.1270.60. Arfaptin. 1 hit.
Pfam	PF06456. Arfaptin. 1 hit. [Graphical view]
PROSITE	PS50870. AH. 1 hit. [Graphical view]
ProDom	P53367. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ARFP1_HUMAN

Accession

Primary (citable) accession number: P53367
Secondary accession number(s): Q3SYL4, Q9Y2X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 27, 2002
Last modified:	September 2, 2008
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)