Reviewed, UniProtKB/Swiss-Prot P54786 (ZDS2_YEAST)

Names and origin

Protein names

Recommended name:
    Protein ZDS2

Gene names

Name:	ZDS2
Synonyms:	MCS1
Ordered Locus Names:	YML109W
ORF Names:	YM8339.10

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a negative regulator of polarized growth via an alternative mechanism to ZDS1. In heat-stressed cells appears to play a role in localizing BCY1 to the cytoplasm. Seems to interact with, and downregulate, CDC42. Also acts as a suppressor of PKC1. May act as an integration point for distinct signaling pathways helping to maintain a balance among these different pathways.
Miscellaneous	'ZDS' means 'zillion different screens' as both ZDS1 and ZDS2 have been found by a wide variety of genetic screens. Present with 105 molecules/cell in log phase SD medium.
Sequence similarities	To yeast ZDS1/NRC1/CES1.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell aging Inferred from mutant phenotype. Source: SGD chromatin silencing at rDNA Inferred from mutant phenotype. Source: SGD establishment of cell polarity Inferred from mutant phenotype. Source: SGD
Cellular component	nucleus Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC55	Q00362	1	EBI-29637,EBI-1942

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 942	942	Protein ZDS2
Amino acid modifications
	Modified residue	50	1	Phosphoserine
	Modified residue	167	1	Phosphoserine
	Modified residue	172	1	Phosphoserine
	Modified residue	716	1	Phosphoserine
Experimental info
	Sequence conflict	7	1	M → V in AAB37541. Ref.1
	Sequence conflict	23	1	T → S in AAB37541. Ref.1
	Sequence conflict	530	1	A → P in AAB37541. Ref.1
	Sequence conflict	546	1	A → G in AAB37541. Ref.1
	Sequence conflict	633	1	V → A in AAB37541. Ref.1
	Sequence conflict	668	1	N → S in AAB37541. Ref.1
	Sequence conflict	711	1	V → VNC in AAB37541. Ref.1
	Sequence conflict	723	1	Missing in AAB37541. Ref.1
	Sequence conflict	729	1	A → E in AAB37541. Ref.1
	Sequence conflict	833	1	S → P in AAB37541. Ref.1
	Sequence conflict	914 – 942	29	TGDIA…LIPDI → HWRYSLQW in AAB37541. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54786-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: A1C9DD9A539E4291	FASTA	942	105,496
	10 20 30 40 50 60 MVLMEDMQNK DGHNTVENSS GGTDSNNNIQ MRRMRKTQLS KKELFEKRKS DVLIAAKSLD 70 80 90 100 110 120 TEIQNVKNLK RLSIGSMDLV IDPELEFKVN SRNSYSSDSS KESLQESLHE ENIIRSEQKE 130 140 150 160 170 180 EQGSEDNDAY EEGDATNVDD SIDITQTEYL HDEETLEKEK IIRNASSSTS SSARVTSRNR 190 200 210 220 230 240 RLSGVKTLAH DVVLDVENDH DSKMVDLTQN LLWVPADQHP NVKPENYLEL IQDTLQNIQI 250 260 270 280 290 300 STNQDIDENK LELGNNHVIS NRKRTGSVVR RPSRLKTSYT KFDDEPPLAD KPQEGEIQVD 310 320 330 340 350 360 KRISSSDIKT IRSVSLKEIT EELTKISNNA GLTDSDAVTL ARSLSMSGSF TNESLHLNGN 370 380 390 400 410 420 HTENDNEFAS NMFNETGLTI PERSSLRRSK FNTYKIRLEG SSLPQAVKLN SLMNIQTNDN 430 440 450 460 470 480 RRSASSPASY TQVPQEQASL NDFHEIFDHY RRTSTDWSTE NEKYVDSTNY YSDEEDLTHA 490 500 510 520 530 540 SISQESSLLS TDSSNNSVLI KPHNTGSMIS EKLDQHVSSS EKSNTNNSEA NHGWSWLNSS 550 560 570 580 590 600 NGSLNANEQT YQQLTDDEDD EECVDNEKAD FVNLSVSRRA KSTKRASERI NHSKNRHSPI 610 620 630 640 650 660 FQIHSEEAKS VVITPSVVSS SESQPSKPTA PAVVEKKVEL PTDTQASTHK KNSLEKRLAK 670 680 690 700 710 720 LFKRKQHNGT CKSDVKVIKK SVKKELKKKA SHSSLSKFRK SPKKKPQEAE VERPSSPTKT 730 740 750 760 770 780 ITTEDIDTAS VIEPEVRSSN ASTLLPDSHT SHSSEFVVET ISELDGDDSF DISGGDVNYD 790 800 810 820 830 840 VEVHSSISRD TTAGLEEDIG AEREDNTSPT APQISTLPPR KLTFEDVVKP DYSNAPIKFT 850 860 870 880 890 900 DSAFGFPLPM ITNSTVIMFD HRLGINVERA IYRLSHLKLS DPGRELRQQV LLSNFMYSYL 910 920 930 940 NLVNHTLYME QVGTGDIAFN GDSALGMMDK NDSDGTILIP DI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mutations in the homologous ZDS1 and ZDS2 genes affect cell cycle progression." Yu Y., Jiang Y.W., Wellinger R.J., Carlson K., Roberts J.M., Stillman D.J. Mol. Cell. Biol. 16:5254-5263(1996) [PubMed: 8816438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Feedback inhibition on cell wall integrity signaling by Zds1 involves Gsk3 phosphorylation of a cAMP-dependent protein kinase regulatory subunit." Griffioen G., Swinnen S., Thevelein J.M. J. Biol. Chem. 278:23460-23471(2003) [PubMed: 12704202] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Pkc1 acts through Zds1 and Gic1 to suppress growth and cell polarity defects of a yeast eIF5A mutant." Zanelli C.F., Valentini S.R. Genetics 171:1571-1581(2005) [PubMed: 16157662] [Abstract] Cited for: FUNCTION.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-716, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-172 AND SER-716, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U32938 Genomic DNA. Translation: AAB37541.1. Z49210 Genomic DNA. Translation: CAA89109.1.
PIR	S53963.
RefSeq	NP_013598.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1517N.
IntAct	P54786.
Genome annotation databases
Ensembl	YML109W. Saccharomyces cerevisiae. [Contig view]
GeneID	854931.
GenomeReviews	Gene locus YML109W in contig Z71257_GR.
KEGG	sce:YML109W.
NMPDR	fig|4932.3.peg.4634.
Organism-specific databases
CYGD	YML109w.
SGD	S000004577. ZDS2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P54786.
Gene expression databases
ArrayExpress	P54786.
GermOnline	YML109W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013941. Spore_Zds1_C. [Graphical view]
Pfam	PF08632. Zds1_C. 1 hit. [Graphical view]
ProDom	P54786. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P54786.
ProtoNet	Search...

Entry information

Entry name

ZDS2_YEAST

Accession

Primary (citable) accession number: P54786

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)