Reviewed, UniProtKB/Swiss-Prot P54793 (ARSF_HUMAN)

Names and origin

Protein names

Recommended name:
    Arylsulfatase F
      Short name=ASF
    EC=3.1.6.-

Gene names

Name:

ARSF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 calcium ion per subunit By similarity.
Enzyme regulation	Not inhibited by DHEAS or warfarin.
Subcellular location	SecretedPotential.
Post-translational modification	The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity By similarity.
Sequence similarities	Belongs to the sulfatase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Molecular function	arylsulfatase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 590	568	Arylsulfatase F
Sites
	Active site	139	1	By similarity
	Metal binding	38	1	Calcium By similarity
	Metal binding	39	1	Calcium By similarity
	Metal binding	78	1	Calcium; via 3-oxoalanine By similarity
	Metal binding	345	1	Calcium By similarity
	Metal binding	346	1	Calcium By similarity
Amino acid modifications
	Modified residue	78	1	3-oxoalanine (Cys) By similarity
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	20 – 29	10	CQAHRVHDDK → WPGHTGCMTTR in CAA66462. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P54793-1 [UniParc]. Last modified October 11, 2005. Version 3. Checksum: F02B6823B40AE78B	FASTA	590	65,966
	10 20 30 40 50 60 MRPRRPLVFM SLVCALLNTC QAHRVHDDKP NIVLIMVDDL GIGDLGCYGN DTMRTPHIDR 70 80 90 100 110 120 LAREGVRLTQ HISAASLCSP SRSAFLTGRY PIRSGMVSSG NRRVIQNLAV PAGLPLNETT 130 140 150 160 170 180 LAALLKKQGY STGLIGKWHQ GLNCDSRSDQ CHHPYNYGFD YYYGMPFTLV DSCWPDPSRN 190 200 210 220 230 240 TELAFESQLW LCVQLVAIAI LTLTFGKLSG WVSVPWLLIF SMILFIFLLG YAWFSSHTSP 250 260 270 280 290 300 LYWDCLLMRG HEITEQPMKA ERAGSIMVKE AISFLERHSK ETFLLFFSFL HVHTPLPTTD 310 320 330 340 350 360 DFTGTSKHGL YGDNVEEMDS MVGKILDAID DFGLRNNTLV YFTSDHGGHL EARRGHAQLG 370 380 390 400 410 420 GWNGIYKGGK GMGGWEGGIR VPGIVRWPGK VPAGRLIKEP TSLMDILPTV ASVSGGSLPQ 430 440 450 460 470 480 DRVIDGRDLM PLLQGNVRHS EHEFLFHYCG SYLHAVRWIP KDDSGSVWKA HYVTPVFQPP 490 500 510 520 530 540 ASGGCYVTSL CRCFGEQVTY HNPPLLFDLS RDPSESTPLT PATEPLYDFV IKKVANALKE 550 560 570 580 590 HQETIVPVTY QLSELNQGRT WLKPCCGVFP FCLCDKEEEV SQPRGPNEKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification by shotgun sequencing, genomic organization, and functional analysis of a fourth arylsulfatase gene (ARSF) from the Xp22.3 region." Puca A.A., Zollo M., Repetto M., Andolfi G., Guffanti A., Simon G., Ballabio A., Franco B. Genomics 42:192-199(1997) [PubMed: 9192838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"A cluster of sulfatase genes on Xp22.3: mutations in chondrodysplasia punctata (CDPX) and implications for warfarin embryopathy." Franco B., Meroni G., Parenti G., Levilliers J., Bernard L., Gebbia M., Cox L., Maroteaux P., Sheffield L., Rappold G.A., Andria G., Petit C., Ballabio A. Cell 81:15-25(1995) [PubMed: 7720070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 369-422. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97868 mRNA. Translation: CAA66462.1. BC022389 mRNA. Translation: AAH22389.1.
PIR	A56217.
RefSeq	NP_004033.2.
UniGene	Hs.101674
3D structure databases
HSSP	HSSP built from PDB template 1P49 based on UniProtKB P08842.
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000062096. Homo sapiens. [Contig view]
GeneID	416.
KEGG	hsa:416.
Organism-specific databases
HGNC	HGNC:721. ARSF.
MIM	300003. gene.
PharmGKB	PA25012.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P54793.
HOVERGEN	P54793.
Gene expression databases
ArrayExpress	P54793.
CleanEx	HS_ARSF.
GermOnline	ENSG00000062096. Homo sapiens.
Family and domain databases
InterPro	IPR017849. Alkaline_Pase-like_a/b/a. IPR000917. Sulphatase. [Graphical view]
Gene3D	G3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
Pfam	PF00884. Sulfatase. 1 hit. [Graphical view]
PROSITE	PS00523. SULFATASE_1. 1 hit. PS00149. SULFATASE_2. 1 hit. [Graphical view]
ProDom	P54793. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

ARSF_HUMAN

Accession

Primary (citable) accession number: P54793
Secondary accession number(s): Q8TCC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 11, 2005
Last modified:	September 2, 2008
This is version 63 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)