Reviewed, UniProtKB/Swiss-Prot P54838 (DAK1_YEAST)

Names and origin

Protein names

Recommended name:
    Dihydroxyacetone kinase 1
      Short name(s)=DHA kinase 1
    EC=2.7.1.29
Alternative name(s):
    Glycerone kinase 1

Gene names

Name:	DAK1
Ordered Locus Names:	YML070W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + glycerone = ADP + glycerone phosphate.
Pathway	Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 2/2.
Miscellaneous	Present with 23600 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the dihydroxyacetone kinase (DAK) family. Contains 1 DAK1 (dihydroxyacetone kinase subunit 1) domain. Contains 1 DAK2 (dihydroxyacetone kinase subunit 2) domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to stress Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	glycerone kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 584	584	Dihydroxyacetone kinase 1
Regions
	Domain	16 – 344	329	DAK1
	Domain	412 – 582	171	DAK2
Amino acid modifications
	Modified residue	5	1	Phosphoserine
	Modified residue	25	1	Phosphoserine
	Modified residue	233	1	Phosphoserine
	Modified residue	365	1	Phosphoserine
	Modified residue	563	1	Phosphoserine

Sequences

	Sequence		Length	Mass (Da)
	P54838-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3B488A5649FAFE25	FASTA	584	62,207
	10 20 30 40 50 60 MSAKSFEVTD PVNSSLKGFA LANPSITLVP EEKILFRKTD SDKIALISGG GSGHEPTHAG 70 80 90 100 110 120 FIGKGMLSGA VVGEIFASPS TKQILNAIRL VNENASGVLL IVKNYTGDVL HFGLSAERAR 130 140 150 160 170 180 ALGINCRVAV IGDDVAVGRE KGGMVGRRAL AGTVLVHKIV GAFAEEYSSK YGLDGTAKVA 190 200 210 220 230 240 KIINDNLVTI GSSLDHCKVP GRKFESELNE KQMELGMGIH NEPGVKVLDP IPSTEDLISK 250 260 270 280 290 300 YMLPKLLDPN DKDRAFVKFD EDDEVVLLVN NLGGVSNFVI SSITSKTTDF LKENYNITPV 310 320 330 340 350 360 QTIAGTLMTS FNGNGFSITL LNATKATKAL QSDFEEIKSV LDLLNAFTNA PGWPIADFEK 370 380 390 400 410 420 TSAPSVNDDL LHNEVTAKAV GTYDFDKFAE WMKSGAEQVI KSEPHITELD NQVGDGDCGY 430 440 450 460 470 480 TLVAGVKGIT ENLDKLSKDS LSQAVAQISD FIEGSMGGTS GGLYSILLSG FSHGLIQVCK 490 500 510 520 530 540 SKDEPVTKEI VAKSLGIALD TLYKYTKARK GSSTMIDALE PFVKEFTASK DFNKAVKAAE 550 560 570 580 EGAKSTATFE AKFGRASYVG DSSQVEDPGA VGLCEFLKGV QSAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Metabolic and regulatory changes associated with growth of Saccharomyces cerevisiae in 1.4 M NaCl. Evidence for osmotic induction of glycerol dissimilation via the dihydroxyacetone pathway." Norbeck J., Blomberg A. J. Biol. Chem. 272:5544-5554(1997) [PubMed: 9038161] [Abstract] Cited for: PROTEIN SEQUENCE OF 104-112 AND 159-167, PARTIAL CHARACTERIZATION. Strain: ATCC 44827 / SKQ2N.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 0:0-0(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-25; SER-233; SER-365 AND SER-563, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z38114 Genomic DNA. Translation: CAA86250.1.
PIR	S48327.
RefSeq	NP_013641.1.
3D structure databases
HSSP	HSSP built from PDB template 1OI2 based on UniProtKB P76015.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4531N.
IntAct	P54838.
Proteomic databases
PeptideAtlas	P54838.
Genome annotation databases
Ensembl	YML070W. Saccharomyces cerevisiae. [Contig view]
GeneID	854932.
GenomeReviews	Gene locus YML070W in contig Z71257_GR.
KEGG	sce:YML070W.
NMPDR	fig|4932.3.peg.4678.
Organism-specific databases
CYGD	YML070w.
SGD	S000004535. DAK1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P54838.
Gene expression databases
ArrayExpress	P54838.
GermOnline	YML070W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004006. Dak1. IPR004007. Dak2. IPR012734. DhaK_ATP. [Graphical view]
Pfam	PF02733. Dak1. 1 hit. PF02734. Dak2. 1 hit. [Graphical view]
TIGRFAMs	TIGR02361. dak_ATP. 1 hit.
ProDom	P54838. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	P54838.
ProtoNet	Search...

Entry information

Entry name

DAK1_YEAST

Accession

Primary (citable) accession number: P54838

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	July 22, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)