Reviewed, UniProtKB/Swiss-Prot P55943 (MT2H_BOVIN)

Names and origin

Protein names	Recommended name:     Metallothionein-II, hippocampal Alternative name(s):     MT-2
Organism	Bos taurus (Bovine)
Taxonomic identifier	9913 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. This isoform may play a role in regulating the transport, accumulation, and compartmentation of zinc in the hippocampus.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 61	61	Metallothionein-II, hippocampal
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Cluster B
	Metal binding	7	1	Cluster B
	Metal binding	13	1	Cluster B
	Metal binding	15	1	Cluster B
	Metal binding	19	1	Cluster B
	Metal binding	21	1	Cluster B
	Metal binding	24	1	Cluster B
	Metal binding	26	1	Cluster B
	Metal binding	29	1	Cluster B
	Metal binding	33	1	Cluster A
	Metal binding	34	1	Cluster A
	Metal binding	36	1	Cluster A
	Metal binding	37	1	Cluster A
	Metal binding	41	1	Cluster A
	Metal binding	44	1	Cluster A
	Metal binding	48	1	Cluster A
	Metal binding	50	1	Cluster A
	Metal binding	57	1	Cluster A
	Metal binding	59	1	Cluster A
	Metal binding	60	1	Cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine

Sequences

	Sequence		Length	Mass (Da)
	P55943-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: DA474940AD2BCB73	FASTA	61	5,956
	10 20 30 40 50 60 MDPNCSCATG GSCTCANSCT CKACKCASCK KSCCSCCPVG CAKCAQGCIC KGASDKCSCC A

References

[1]	"Amino acid composition, immunoreactivity, sequence analysis, and function of bovine hippocampal metallothionein isoforms." Ebadi M., Perini F., Mountjoy K., Garvey J.S. J. Neurochem. 66:2121-2127(1996) [PubMed: 8780044] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Hippocampus.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1MHU based on UniProtKB P02795.
SMR	P55943. Positions 1-61.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000038067. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	P55943.
Family and domain databases
InterPro	IPR003019. Metallothionein_sfam_euk. IPR000006. Metallothionein_vert. [Graphical view]
Gene3D	G3DSA:4.10.10.10. Metallothionein_vert. 1 hit.
PANTHER	PTHR23299. Metallothionein_vert. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProDom	P55943. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MT2H_BOVIN

Accession

Primary (citable) accession number: P55943

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 2, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)