Reviewed, UniProtKB/Swiss-Prot P55956 (ASP3_CAEEL)

Names and origin

Protein names

Recommended name:
    Aspartic protease 3
    EC=3.4.23.-

Gene names

Name:	asp-3
ORF Names:	H22K11.1

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell death Inferred from mutant phenotype. Source: WormBase determination of adult life span Inferred from mutant phenotype. Source: WormBase
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
erm-1	P91016	1	EBI-313689,EBI-313664

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 55	38	Removed in mature form
	Chain	56 – 398	343	Aspartic protease 3
Sites
	Active site	87	1	By similarity
	Active site	279	1	By similarity
Amino acid modifications
	Glycosylation	321	1	N-linked (GlcNAc...)
	Disulfide bond	100 ↔ 107		By similarity
	Disulfide bond	313 ↔ 351		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P55956-1 [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 4A16251D2CB14121	FASTA	398	43,413
	10 20 30 40 50 60 MSGRVFLLLA LVALASAIQR IKLEKRTYTR EQYKFGSIQE HLKAKYVPGY IPNKDAFNEG 70 80 90 100 110 120 LSDYSNAQYY GPVTIGTPPQ NFQVLFDTGS SNLWVPCANC PFGDIACRMH NRFDCKKSSS 130 140 150 160 170 180 CTATGASFEI QYGTGSMKGT VDNDVVCFGH DTTYCTDKNQ GLACATSEPG ITFVAAKFDG 190 200 210 220 230 240 IFGMGWDTIS VNKISQPMDQ IFANSAICKN QLFAFWLSRD ANDITNGGEI TLCETDPNHY 250 260 270 280 290 300 VGNIAWEPLV SEDYWRIKLA SVVIDGTTYT SGPIDSIVDT GTSLLTGPTD VIKKIQHKIG 310 320 330 340 350 360 GIPLFNGEYE VECSKIPSLP NITFNLGGQN FDLQGKDYIL QMSNGNGGST CLSGFMGMDI 370 380 390 PAPAGPLWIL GDVFIGRFYS VFDHGNKRVG FATSRTGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[2]	"Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing." Bini L., Heid H., Liberatori S., Geier G., Pallini V., Zwilling R. Electrophoresis 18:557-562(1997) [PubMed: 9150941] [Abstract] Cited for: PROTEIN SEQUENCE OF 56-65. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-321, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF077544 Genomic DNA. Translation: AAK39240.1.
PIR	T33383.
RefSeq	NP_509142.1.
UniGene	Cel.17775
3D structure databases
HSSP	HSSP built from PDB template 4CMS based on UniProtKB P00794.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:26574N.
IntAct	P55956.
2-D gel databases
Siena-2DPAGE	P55956.
Genome annotation databases
Ensembl	H22K11.1. Caenorhabditis elegans. [Contig view]
GeneID	180947.
KEGG	cel:H22K11.1.
NMPDR	fig|6239.3.peg.23623.
Organism-specific databases
WormBase	WBGene00000216. asp-3.
WormPep	H22K11.1. CE19495. [WorfDB]
Gene expression databases
ArrayExpress	P55956.
Family and domain databases
InterPro	IPR001969. Pept_Asp_AS. IPR009007. Pept_Aspartc_cat. IPR001461. Peptidase_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 1 hit. [Graphical view]
ProDom	P55956. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ASP3_CAEEL

Accession

Primary (citable) accession number: P55956
Secondary accession number(s): Q9TXI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 20, 2002
Last modified:	July 22, 2008
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project