Reviewed, UniProtKB/Swiss-Prot P56064 (PRIM_HELPY)

Names and origin

Protein names

Recommended name:
    DNA primase
    EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	HP_0012

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 559	559	DNA primase
Regions
	Zinc finger	37 – 61	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56064-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E5EB7F2F0BB38170	FASTA	559	63,723
	10 20 30 40 50 60 MILKSSIDRL LQTIDIVEVI SSYVNLRKSG SSYMACCPFH EERSASFSVN QIKGFYHCFG 70 80 90 100 110 120 CGASGDSIKF VMAFEKLSFV EALEKLAHRF NIVLEYDKGV YYDHKEDYHL LEMVSSLYQE 130 140 150 160 170 180 ELFNAPFFLN YLQKRGLSLE SIKAFKLGLC TNRIDYGIEN KGLNKDKLIE LGVLGKSDND 190 200 210 220 230 240 QKTYLRFLDR IMFPIYSPSA QVVGFGGRTL KEKAAKYINS PQSKLFDKSS LLYGYHLAKE 250 260 270 280 290 300 HIYKQKQVIV TEGYLDVILL HQAGFKNAIA TLGTALTPSH LPLLKKGDPE ILLSYDGDKA 310 320 330 340 350 360 GRNAAYKASL MLAKEQRRGG VILFENNLDP ADMIANGQIE TLKNWLSHPM AFIEFVLRRM 370 380 390 400 410 420 ADSYLLDDPL EKDKALKEML GFLKNFSLLL QSEYKPLIAT LLQAPLHVLG IRERVSFQPF 430 440 450 460 470 480 YPKTEKPNRP QRFAHVSSAP SLEFLEKLVI RYLLEDRSLL DLAVGYIHSG VFLHKKQEFD 490 500 510 520 530 540 ALCQEKLDDP KLVALLLDAN LPLKKGGFEK ELRLLILRYF ERQLKEIPKS SLPFSEKMIC 550 LKKARQAIMK LKQGELVAI

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07082.1.
PIR	D64521.
RefSeq	NP_206814.1.
3D structure databases
HSSP	HSSP built from PDB template 1D0Q based on UniProtKB Q9X4D0.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3301N.
Genome annotation databases
GeneID	899091.
GenomeReviews	Gene locus HP_0012 in contig AE000511_GR.
KEGG	hpy:HP0012.
NMPDR	fig|85962.1.peg.12.
TIGR	HP_0012.
Phylogenomic databases
HOGENOM	P56064.
Family and domain databases
InterPro	IPR013264. DNA_primase_core_N. IPR006295. DNA_primase_DnaG. IPR006171. Toprim_dom. IPR006154. Toprim_sub. IPR002694. Znf_CHC2. [Graphical view]
Gene3D	G3DSA:3.90.980.10. Toprim_N. 1 hit. G3DSA:3.90.580.10. Znf_CHC2. 1 hit.
Pfam	PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
ProDom	PD002276. Toprim_primase. 1 hit. PD002988. Znf_CHC2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. dnaG. 1 hit.
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PRIM_HELPY

Accession

Primary (citable) accession number: P56064

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)