Reviewed, UniProtKB/Swiss-Prot P56071 (SYT_HELPY)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	HP_0123

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 612	612	Threonyl-tRNA synthetase
Regions
	Region	218 – 509	292	Catalytic
Sites
	Metal binding	310	1	Zinc; catalytic By similarity
	Metal binding	361	1	Zinc; catalytic By similarity
	Metal binding	486	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56071-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 47FEF701E389AC01	FASTA	612	70,240
	10 20 30 40 50 60 MSAELIAVYK DEQIIDLESA KVLGLSDGIK ALNGTEPIYF DDSPLALEVI RHSCAHLLAQ 70 80 90 100 110 120 SLKALYPDAK FFVGPVVEEG FYYDFKTSSK ISEEDLPKIE AKMKEFAKLK LAITKETLTR 130 140 150 160 170 180 EQALERFKGD ELKHAVMSKI GGDAFGVYQQ GEFEDLCKGP HLPNTRFLNH FKLTKLAGAY 190 200 210 220 230 240 LGGDENNEML IRIYGIAFAT KEGLKDYLFQ IEEAKKRDHR KLGVELGLFS FDDEIGAGLP 250 260 270 280 290 300 LWLPKGARLR KRIEDLLSQA LLLRGYEPVK GPEILKSDVW KISGHYDNYK ENMYFTTIDE 310 320 330 340 350 360 QEYGIKPMNC VGHIKVYQSA LHSYRDLPLR FYEYGVVHRH EKSGVLHGLL RVREFTQDDA 370 380 390 400 410 420 HIFCSFEQIQ SEVSAILDFT HKIMQAFDFS YEMELSTRPA KSIGDDKVWE KATNALKEAL 430 440 450 460 470 480 KEHRIDYKID EGGGAFYGPK IDIKITDALK RKWQCGTIQV DMNLPERFKL AFTNEYNHAE 490 500 510 520 530 540 QPVMIHRAIL GSFERFIAIL SEHFGGNFPF FVAPTQIALI PINEEHHVFA LKLKEALKKR 550 560 570 580 590 600 DIFVEVLDKN DSLNKKVRLA EKQKIPMILV LGNEEVETEI LSIRDREKQD QYKMPLKEFL 610 NMVESKMQEV SF

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07192.1.
PIR	C64535.
RefSeq	NP_206923.1.
3D structure databases
HSSP	HSSP built from PDB template 1EVL based on UniProtKB P00955.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3171N.
Genome annotation databases
GeneID	900156.
GenomeReviews	Gene locus HP_0123 in contig AE000511_GR.
KEGG	hpy:HP0123.
NMPDR	fig|85962.1.peg.121.
TIGR	HP_0123.
Phylogenomic databases
HOGENOM	P56071.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon_bd. IPR002320. Thr-tRNA-synth_IIa. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProDom	P56071. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYT_HELPY

Accession

Primary (citable) accession number: P56071

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 2, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)