Reviewed, UniProtKB/Swiss-Prot P56073 (AROK_HELPY)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	HP_0157

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 5/7. Context: Aromatic amino acids biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the shikimate kinase family.
Biophysicochemical properties	Kinetic parameters: KM=60 µM for shikimate KM=101 µM for MgATP Vmax=22 µmol/min/mg enzyme toward shikimate Vmax=26 µmol/min/mg enzyme toward MgATP

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 162

162

Shikimate kinase

Regions

Nucleotide binding

11 – 16

6

ATP By similarity

Region

109 – 123

15

LID domain

Sites

Metal binding

15

1

Magnesium By similarity

Binding site

33

1

Substrate

Binding site

57

1

Substrate

Binding site

80

1

Substrate; via amide nitrogen

Binding site

116

1

ATP By similarity

Binding site

132

1

Substrate

Secondary structure

1

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162

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Helix

14 – 25

12

Beta strand

29 – 31

3

Helix

32 – 40

9

Helix

44 – 50

7

Helix

53 – 68

16

Beta strand

75 – 77

3

Helix

80 – 84

5

Helix

86 – 88

3

Beta strand

91 – 98

8

Helix

101 – 108

8

Helix

123 – 138

16

Beta strand

141 – 145

5

Helix

146 – 148

3

Helix

150 – 159

10

Sequences

	Sequence		Length	Mass (Da)
	P56073-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3D4B5F74E1C958FE	FASTA	162	18,411
	10 20 30 40 50 60 MQHLVLIGFM GSGKSSLAQE LGLALKLEVL DTDMIISERV GLSVREIFEE LGEDNFRMFE 70 80 90 100 110 120 KNLIDELKTL KTPHVISTGG GIVMHENLKG LGTTFYLKMD FETLIKRLNQ KEREKRPLLN 130 140 150 160 NLTQAKELFE KRQALYEKNA SFIIDARGGL NNSLKQVLQF IA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	"Discovery of Helicobacter pylori shikimate kinase inhibitors: bioassay and molecular modeling." Han C., Zhang J., Chen L., Chen K., Shen X., Jiang H. Bioorg. Med. Chem. 15:656-662(2007) [PubMed: 17098431] [Abstract] Cited for: INHIBITORS.
[3]	"Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase." Cheng W.-C., Chang Y.-N., Wang W.-C. J. Bacteriol. 187:8156-8163(2005) [PubMed: 16291688] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF APOENYZME AND IN COMPLEX WITH SHIKIMATE, FUNCTION, KINETIC PARAMETERS. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000511 Genomic DNA. Translation: AAD07220.1.

PIR

E64539.

RefSeq

NP_206956.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUH	X-ray	1.80	A	1-162	[»]
1ZUI	X-ray	2.30	A	1-162	[»]

ModBase

Search...

Genome annotation databases

GeneID

898985.

GenomeReviews

Gene locus HP_0157 in contig AE000511_GR.

KEGG

hpy:HP0157.

NMPDR

fig|85962.1.peg.154.

TIGR

HP_0157.

Phylogenomic databases

HOGENOM

P56073.

Family and domain databases

HAMAP

MF_00109.
[Tree]

InterPro

IPR000623. Shik_kinase.
[Graphical view]

Pfam

PF01202. SKI. 1 hit.
[Graphical view]

PRINTS

PR01100. SHIKIMTKNASE.

PROSITE

PS01128. SHIKIMATE_KINASE. 1 hit.
[Graphical view]

ProDom

P56073.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

AROK_HELPY

Accession

Primary (citable) accession number: P56073

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)