Reviewed, UniProtKB/Swiss-Prot P56126 (SYK_HELPY)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	HP_0182

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 501	501	Lysyl-tRNA synthetase
Sites
	Metal binding	402	1	Magnesium 1 By similarity
	Metal binding	409	1	Magnesium 1 By similarity
	Metal binding	409	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56126-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 594EF69873E9428E	FASTA	501	57,782
	10 20 30 40 50 60 MFSNQYIQQR IHKANSLREE GKNPYQNGLK RSLTNAAFLE KYAYVKGLEE PKDKEKCESI 70 80 90 100 110 120 VGRVKLLRLM GKACFIKVED ESTILQVYVS QNELNDEFKS LKKHLEVGDI VLVKGFPFAT 130 140 150 160 170 180 KTGELSIHAL EFHILSKTIV PLPEKFHGLS DIELRYRQRY LDLIVNPSVK DVFKKRSLIV 190 200 210 220 230 240 SSVRKFFEME GFLEVETPMM HPIPGGANAR PFITYHNALE VERYLRIAPE LYLKRLIVGG 250 260 270 280 290 300 FEAVFEINRN FRNEGMDHSH NPEFTMIEFY WAYHTYEDLI ELSKRLFDYL LKTLNLDSKI 310 320 330 340 350 360 IYNDMEVDFN QTSVISYLDA LETIGGISKD ILEKEDRLLA YLLEQGIKVE PNLTYGKLLA 370 380 390 400 410 420 EAFDHFVEHQ LINPTFVTQY PIEISPLARR NDSNPNIADR FELFIAGKEI ANGFSELNDP 430 440 450 460 470 480 LDQLERFKNQ VAEKEKGDEE AQYMDEDYVW ALAHGMPPTA GQGIGIDRLV MLLTGAKSIK 490 500 DVILFPAMRP VKNDFNVESE E

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07251.1.
PIR	F64542.
RefSeq	NP_206981.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3629N.
Genome annotation databases
GeneID	900250.
GenomeReviews	Gene locus HP_0182 in contig AE000511_GR.
KEGG	hpy:HP0182.
NMPDR	fig|85962.1.peg.179.
TIGR	HP_0182.
Phylogenomic databases
HOGENOM	P56126.
Family and domain databases
HAMAP	MF_00252. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-synth_II. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProDom	P56126. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYK_HELPY

Accession

Primary (citable) accession number: P56126

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)