Reviewed, UniProtKB/Swiss-Prot P56138 (MNMG_HELPY)

Names and origin

Protein names

Recommended name:
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Alternative name(s):
    Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	HP_0213

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 By similarity.
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the mnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 621	621	tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity
	Nucleotide binding	270 – 284	15	NAD Potential
Natural variations
	Natural variant	10	1	V → I in strain: 60190.
	Natural variant	105	1	L → F in strain: 60190.
	Natural variant	193	1	D → E in strain: 60190.
	Natural variant	220	1	T → A in strain: 60190.
	Natural variant	302	1	S → N in strain: 60190.
	Natural variant	353	1	T → A in strain: 60190.
	Natural variant	379	1	A → D in strain: 60190.
	Natural variant	415	1	I → V in strain: 60190.
	Natural variant	457	1	E → Q in strain: 60190.
	Natural variant	480 – 481	2	YI → CV in strain: 60190.
	Natural variant	487 – 488	2	EV → KL in strain: 60190.
	Natural variant	493	1	D → N in strain: 60190.
	Natural variant	505	1	D → N in strain: 60190.

Sequences

	Sequence		Length	Mass (Da)
	P56138-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C5C5CE80259E49E8	FASTA	621	69,684
	10 20 30 40 50 60 MVKESDILVV GGGHAGIEAS LIAAKMGARV HLITMLIDTI GLASCNPAIG GLGKGHLTKE 70 80 90 100 110 120 VDVLGGAMGI ITDHSGLQYR VLNASKGPAV RGTRAQIDMD TYRILARNLV LNTPNLSVSQ 130 140 150 160 170 180 EMTESLILEN DEVVGVTTNI NNTYRAKKVI ITTGTFLKGV VHIGEHQNQN GRFGENASNS 190 200 210 220 230 240 LALNLRELGF KVDRLKTGTC PRVAGNSIDF EGLEEHFGDT NPPYFSYKTK DFNPTQLSCF 250 260 270 280 290 300 ITYTNPITHQ IIRDNFHRAP LFSGQIEGIG PRYCPSIEDK INRFSEKERH QLFLEPQTIH 310 320 330 340 350 360 KSEYYINGLS TSLPLDVQEK VIHSIKGLEN ALITRYGYAI EYDFIQPTEL THTLETKKIK 370 380 390 400 410 420 GLYLAGQING TTGYEEAAAQ GLMAGINAVL ALKNQAPFIL KRNEAYIGVL IDDLITKGTN 430 440 450 460 470 480 EPYRMFTSRA EYRLLLREDN TLFRLGEHAY RLGLMEEDFY KELKKDKQEI QDNLKRLKEY 490 500 510 520 530 540 ILTPSKEVLK RLDELDENPI NDKVDGVSLL ARDSFNAEKM RSFFSFLAPL NERVLEQIKI 550 560 570 580 590 600 ECKYNIYIEK QHENIAKMDS MLKVSIPKGF VFKGIPGLSL EAVEKLEKFR PKSLFEASEI 610 620 SGITPANLDV LHLYIHLRKN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[2]	Karita M., Etterbeek M.L., Forsyth M.H., Tummuru M.K.R., Blaser M.J. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49503 / 60190.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000511 Genomic DNA. Translation: AAD07281.1. AF008565 Genomic DNA. Translation: AAB63296.1.
PIR	E64546.
RefSeq	NP_207011.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3538N.
Genome annotation databases
GeneID	898934.
GenomeReviews	Gene locus HP_0213 in contig AE000511_GR.
KEGG	hpy:HP0213.
NMPDR	fig|85962.1.peg.209.
TIGR	HP_0213.
Phylogenomic databases
HOGENOM	P56138.
Family and domain databases
HAMAP	MF_00129. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR002218. GIDA. IPR004416. GidA_sub. IPR001100. Pyr_nuc-diS_OxRdtase. [Graphical view]
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MNMG_HELPY

Accession

Primary (citable) accession number: P56138
Secondary accession number(s): O32632

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 2, 2008
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)