Reviewed, UniProtKB/Swiss-Prot P56489 (ACM1_MACMU)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M1

Gene names

Name:

CHRM1

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subunit structure	Interacts with GPRASP2 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 460	460	Muscarinic acetylcholine receptor M1
Regions
	Topological domain	1 – 24	24	Extracellular Potential
	Transmembrane	25 – 47	23	1 Potential
	Topological domain	48 – 61	14	Cytoplasmic Potential
	Transmembrane	62 – 82	21	2 Potential
	Topological domain	83 – 99	17	Extracellular Potential
	Transmembrane	100 – 121	22	3 Potential
	Topological domain	122 – 141	20	Cytoplasmic Potential
	Transmembrane	142 – 164	23	4 Potential
	Topological domain	165 – 186	22	Extracellular Potential
	Transmembrane	187 – 209	23	5 Potential
	Topological domain	210 – 366	157	Cytoplasmic Potential
	Transmembrane	367 – 387	21	6 Potential
	Topological domain	388 – 401	14	Extracellular Potential
	Transmembrane	402 – 421	20	7 Potential
	Topological domain	422 – 460	39	Cytoplasmic Potential
Amino acid modifications
	Modified residue	428	1	Phosphothreonine Potential
	Modified residue	451	1	Phosphoserine Potential
	Modified residue	455	1	Phosphothreonine Potential
	Modified residue	457	1	Phosphoserine Potential
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	12	1	N-linked (GlcNAc...) Potential
	Disulfide bond	98 ↔ 178		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56489-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 1B19845C3BFE79CA	FASTA	460	51,433
	10 20 30 40 50 60 MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN 70 80 90 100 110 120 NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS 130 140 150 160 170 180 FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI 190 200 210 220 230 240 QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS 250 260 270 280 290 300 SERSQPGAEG SPETPPGRCC RCCRPPRLLQ AYSWKEDEEE DEGSMESLTS SEGEEPGSEV 310 320 330 340 350 360 VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA KRKTFSLVKE 370 380 390 400 410 420 KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STINPMCYAL 430 440 450 460 CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC

References

[1]	Rae J.L., Shepard A.R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF026262 mRNA. Translation: AAB95157.1.
RefSeq	NP_001028117.1.
UniGene	Mmu.3424
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMMUG00000017292. Macaca mulatta. [Contig view]
GeneID	574362.
KEGG	mcc:574362.
Phylogenomic databases
HOVERGEN	P56489.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR002228. Musac_M1_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00538. MUSCRINICM1R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P56489. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM1_MACMU

Accession

Primary (citable) accession number: P56489

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	July 22, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)