Reviewed, UniProtKB/Swiss-Prot P56490 (ACM5_MACMU)

Names and origin

Protein names

Recommended name:
    Muscarinic acetylcholine receptor M5

Gene names

Name:

CHRM5

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 532	532	Muscarinic acetylcholine receptor M5
Regions
	Topological domain	1 – 29	29	Extracellular Potential
	Transmembrane	30 – 53	24	1 Potential
	Topological domain	54 – 66	13	Cytoplasmic Potential
	Transmembrane	67 – 87	21	2 Potential
	Topological domain	88 – 104	17	Extracellular Potential
	Transmembrane	105 – 126	22	3 Potential
	Topological domain	127 – 146	20	Cytoplasmic Potential
	Transmembrane	147 – 169	23	4 Potential
	Topological domain	170 – 191	22	Extracellular Potential
	Transmembrane	192 – 214	23	5 Potential
	Topological domain	215 – 443	229	Cytoplasmic Potential
	Transmembrane	444 – 464	21	6 Potential
	Topological domain	465 – 478	14	Extracellular Potential
	Transmembrane	479 – 498	20	7 Potential
	Topological domain	499 – 532	34	Cytoplasmic Potential
Amino acid modifications
	Modified residue	501	1	Phosphothreonine Potential
	Modified residue	505	1	Phosphothreonine Potential
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Disulfide bond	103 ↔ 183		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P56490-1 [UniParc]. Last modified July 15, 1998. Version 1. Checksum: 33BCF6D67E600D79	FASTA	532	60,141
	10 20 30 40 50 60 MEGDSYHNAT TVNGTPVYHQ PLERHRLWEV ISIAAVTAVV SLITIVGNVL VMISFKVNSQ 70 80 90 100 110 120 LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS LACDLWLALD YVASNASVMN 130 140 150 160 170 180 LLVISFDRYF SITRPLTYRA KRTPKRAGVM IGLAWLISFI LWAPAILCWQ YLVGKRTVPL 190 200 210 220 230 240 DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTEAE 250 260 270 280 290 300 KRKPAHRALF RSCLRCPRPT LAQRERNQTS WSSSRRSAST SGKPSQATDP STNQAKAEQL 310 320 330 340 350 360 TTCSSYPSSE DEDKPATDPV LQVVYKSRGK ESPGEEFSSE DAEETFVKAQ TEKHDSDTPN 370 380 390 400 410 420 YFLSPAAAHR PKSQKCVAYK FRLVVKADGT QENNNGCHKV KIMPCSFPVA KEPSTKGLNP 430 440 450 460 470 480 NPSHQMTKRK RMVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG 490 500 510 520 530 YWLCYVNSTV NPICYALCNR TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP

References

[1]	Rae J.L., Shepard A.R. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lens epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF026264 mRNA. Translation: AAB95159.1.
RefSeq	NP_001028103.1.
UniGene	Mmu.10344
3D structure databases
HSSP	HSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMMUG00000014227. Macaca mulatta. [Contig view]
GeneID	574330.
KEGG	mcc:574330.
Phylogenomic databases
HOVERGEN	P56490.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000502. Musac_M5_rcpt. IPR000995. Musac_rcpt. [Graphical view]
PANTHER	PTHR19266:SF36. Musac_M5_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00542. MUSCRINICM5R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	P56490. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ACM5_MACMU

Accession

Primary (citable) accession number: P56490

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 15, 1998
Last modified:	July 22, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)