Reviewed, UniProtKB/Swiss-Prot P57117 (MNMG_BUCAI)

Names and origin

Protein names

Recommended name:
    tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Alternative name(s):
    Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	BU001

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	628 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 By similarity.
Cofactor	FAD By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the mnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 628	628	tRNA uridine 5-carboxymethylaminomethyl modification enzyme mnmG
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity
	Nucleotide binding	273 – 287	15	NAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P57117-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 06E3745332BC077B	FASTA	628	70,307
	10 20 30 40 50 60 MFNLRNFDVI VVGAGHAGTE AAMASSRMGC KTLLLTQKIS DLGALSCNPA IGGIGKSHLV 70 80 90 100 110 120 KEIDALGGMM AKAIDYSGIQ FRILNSSKGP AVRSTRAQAD KILYHETVKK ILKKQNNLLI 130 140 150 160 170 180 LEAEVKDLIF KNYSVVGVLT QNEINFYSRS VVLAAGTFLG GKIHIGLKSY SAGRIGDKSA 190 200 210 220 230 240 IDLSVRLREL SLRVNRLKTG TPPRIDINTV NFNNLLIQNS DTPVPVFSFM GNVSHHPKQI 250 260 270 280 290 300 PCYLTHTNEK THEIIRKNLD KSPIYTGFLK GLGPRYCPSI EDKIVRFPDR KSHQVFLEPE 310 320 330 340 350 360 GLSSIKVYPN GISTSLPIEV QEQIVASIKG LEKSKIIRPG YAIEYDFFDP KDLNLTLESK 370 380 390 400 410 420 LIKGLFFAGQ INGTTGYEEA ASQGLLAGLN AALSSKNTEG WFPRRDQAYL GVLIDDLTTQ 430 440 450 460 470 480 GTEEPYRMFT SRAEYRLSLR EDNADLRLTE IGRKLGLVND SRWIRYNQKV LNIQTEMNRL 490 500 510 520 530 540 KKNKISPISP DADILKKLYN INLIKEISMS ELLKRPQIRY QDLQSLESFR TGIVDLEAIG 550 560 570 580 590 600 QIENEIKYAG YIKRQSEEIE RHLKNENTFL SSIYDYNKIR GLSSEVVKKL NDYKPISIGQ 610 620 ASRISGITPA AISILLIHLK KESYKHTL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12729.1.
RefSeq	NP_239843.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109510.
GenomeReviews	Gene locus BU001 in contig BA000003_GR.
KEGG	buc:BU001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57117.
Enzyme and pathway databases
BioCyc	BSP107806:BU001-MON.
Family and domain databases
HAMAP	MF_00129. [Tree]
InterPro	IPR002218. GIDA. IPR004416. GidA_sub. [Graphical view]
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00136. gidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MNMG_BUCAI

Accession

Primary (citable) accession number: P57117

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	September 2, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)