Reviewed, UniProtKB/Swiss-Prot P57126 (GYRB_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit B
    EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	BU010

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	803 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the type II topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 803	803	DNA gyrase subunit B

Sequences

	Sequence		Length	Mass (Da)
	P57126-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 4CC9D529AF886774	FASTA	803	92,411
	10 20 30 40 50 60 MKNIYDSSNI KILRGLDAVR KRPGMYIGDT DDGSGLHHMV FEIVDNSIDE ALSGYCKEII 70 80 90 100 110 120 VTIHSDNSVS VKDDGRGIPT DIHPEENISA AEVILTVLHS GGKFDNASYQ ISGGLHGVGI 130 140 150 160 170 180 SVVNALSEKL ELIIDKNKKK YQQIYRHGKP ENPLSIIGTT DTTGTYIRFW PSYKTFTNNI 190 200 210 220 230 240 KFQYEILSKR LRELSFLNSN IAIYLEDNRT SIKNCYHYKG GIKAFIKFLN AKKTPIHTHI 250 260 270 280 290 300 FYFRSTKDQI ELEIAMQWNN SHQENILCFT NNIPQKDGGT HLAGFRSGMT RTLNLHIERE 310 320 330 340 350 360 GYNKKNKTTI IGEDVREGLT AIISIKIPDP KFSSQTKDKL VSSEARSVIE SLINENLIEY 370 380 390 400 410 420 LLENPSDSKF IIQKIINAAK VREAARRARE INKKKSILDL GALPGKLSDC QENDPKFSEI 430 440 450 460 470 480 YLVEGDSAGG SAKQGRNRKN QAILPLKGKI LNVEKSKFDK MILSQEVASL ITALGCGIGK 490 500 510 520 530 540 NEYNIDKLRY HYIIIMTDAD VDGAHIRTLL LTFFYRQLPE LIEKGYVYIA QPPLYKLKKG 550 560 570 580 590 600 RKEKYIKNDE EMNTYQIKNA LKDLVLKSED NLIINQKLKK FKKIMSEYHY IQIIMKQSKH 610 620 630 640 650 660 YFPKLVFNEL IYHNHLHNLK DIEIVKEWIY TLAENLNKKD NSNNHYSIEI KENLDKNIFE 670 680 690 700 710 720 PTIKISRYAH YTKYDFKEEF LKSKEYLLIT NLGESFKKYF SDKVLIEKSG STYKIDSIKN 730 740 750 760 770 780 TWKWLIKQSK RGLFIQRYKG LGEMNPEQLW NTTMNPETRN MLQVTIKDAV SANNLFNTLM 790 800 GEAVEPRRQF IENNALKAEN IDV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12738.1.
RefSeq	NP_239852.1.
3D structure databases
HSSP	HSSP built from PDB template 1KZN based on UniProtKB P06982.
ModBase	Search...
Genome annotation databases
GeneID	1109519.
GenomeReviews	Gene locus BU010 in contig BA000003_GR.
KEGG	buc:BU010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57126.
Enzyme and pathway databases
BioCyc	BSP107806:BU010-MON.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR011557. GyrB. IPR001241. Topo_IIA_B/N. IPR013759. Topo_IIA_B/N_ab. IPR013506. Topo_IIA_B_2. IPR002288. Topo_IIA_B_C. IPR006171. Toprim_dom. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
ProDom	PD149633. DNA_gyrase_B. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01059. gyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

GYRB_BUCAI

Accession

Primary (citable) accession number: P57126

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)