Reviewed, UniProtKB/Swiss-Prot P57136 (RSMD_BUCAI)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA small subunit methyltransferase D
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase
    16S rRNA m2G966 methyltransferase

Gene names

Name:	rsmD
Ordered Locus Names:	BU023

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmD family.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 191	191	Ribosomal RNA small subunit methyltransferase D

Sequences

	Sequence		Length	Mass (Da)
	P57136-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 436C5F084F13D420	FASTA	191	22,651
	10 20 30 40 50 60 MNNSFFRKNS KIYIISGNLK GRKISFKNIP NLRPTTNQIR ETLFEWLSKY IKNSRCLDCF 70 80 90 100 110 120 AGSGVLGIEA ISRYAAFSTL LEIEKKTFLT LKKNIKELNI YNVEIIRTNT LHWLKKTRNK 130 140 150 160 170 180 PYDIIFIDPP YHQGLVKKTI NLLENKKWIK KNSFIYIEQE KKQSIIVPKN WTLYKKKITN 190 QIQCYLYICS T

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12750.1.
RefSeq	NP_239864.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109531.
GenomeReviews	Gene locus BU023 in contig BA000003_GR.
KEGG	buc:BU023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57136.
Enzyme and pathway databases
BioCyc	BSP107806:BU023-MON.
Family and domain databases
InterPro	IPR004398. CHP00095. IPR016065. DUF_methylase. IPR002052. N6_adenine_Mtase_CS. [Graphical view]
Pfam	PF03602. Cons_hypoth95. 1 hit. [Graphical view]
PIRSF	PIRSF004553. CHP00095. 1 hit.
TIGRFAMs	TIGR00095. CHP95. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProDom	P57136. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RSMD_BUCAI

Accession

Primary (citable) accession number: P57136

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	September 2, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)