Reviewed, UniProtKB/Swiss-Prot P57143 (PUR9_BUCAI)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including 2 domains:
  Recommended name:
      Phosphoribosylaminoimidazolecarboxamide formyltransferase
      EC=2.1.2.3
  Alternative name(s):
      AICAR transformylase
  Recommended name:
      IMP cyclohydrolase
      EC=3.5.4.10
  Alternative name(s):
      Inosinicase
      IMP synthetase
      ATIC

Gene names

Name:	purH
Ordered Locus Names:	BU031

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 525	525	Bifunctional purine biosynthesis protein purH

Sequences

	Sequence		Length	Mass (Da)
	P57143-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: A89AD962C0152473	FASTA	525	59,054
	10 20 30 40 50 60 MPSNNLIKNA LISVSDKKNI VEVAEKLIIN KINLFSTGGT AQILKKNNIP VTEISDYTKF 70 80 90 100 110 120 PEIMDGRVKT LHPKIMGGIL GQKQKDQEIM KLYNICPIDI VIVNFYPFEK IKNIKKNDID 130 140 150 160 170 180 NVVNNIDIGG PTLVRASAKN YKNVIVIVDL DDFQSTIDSI NNNTMNMEKR FNLASKAFEY 190 200 210 220 230 240 TSYYEQIISQ YFIEQNSLYK KTNNSLFPNE INFSFIKKQD LRYGENYHQK SSFYIEKNMC 250 260 270 280 290 300 DSGTISTACQ IQGKTLSYNN ISDSDIALEC VKQFTKPACV IVKHGNPCSV AVSHNILESY 310 320 330 340 350 360 LSAYNSDPIS AFGGIISFNC KLDEKTAQTI INQQFVEVII IPEISKKAVK ILQKKQNIRV 370 380 390 400 410 420 LVTGKLQNNT VGLDLKKITN GLLVQEYDSH NIDYNSWSFV TKRSPTKKEL KDSIFCWQVA 430 440 450 460 470 480 KFVKSNAIVY GSDEITIGIG AGQMSRIYST KLANIKVKDQ GKNIIGATMA SDAFFPFRDG 490 500 510 520 IDEAASVGIS SIIQPGGSIR DEEIIRAADE HNITMIFTKK RHFKH

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12758.1.
RefSeq	NP_239872.1.
3D structure databases
HSSP	HSSP built from PDB template 1G8M based on UniProtKB P31335.
ModBase	Search...
Genome annotation databases
GeneID	1109539.
GenomeReviews	Gene locus BU031 in contig BA000003_GR.
KEGG	buc:BU031.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57143.
Enzyme and pathway databases
BioCyc	BSP107806:BU031-MON.
Family and domain databases
HAMAP	MF_00139. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProDom	P57143. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PUR9_BUCAI

Accession

Primary (citable) accession number: P57143

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)