Reviewed, UniProtKB/Swiss-Prot P57146 (RPOB_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase subunit beta
      Short name(s)=RNAP subunit beta
    EC=2.7.7.6
Alternative name(s):
    Transcriptase subunit beta
    RNA polymerase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BU034

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	1342 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1342	1342	DNA-directed RNA polymerase subunit beta

Sequences

	Sequence		Length	Mass (Da)
	P57146-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 95252459873DF940	FASTA	1,342	151,468
	10 20 30 40 50 60 MVYSYTEKKR IRKDFGKRPK VLDIPYLLSI QLNSFKKFIQ PDLSGQHGLE AAFRSVFPIR 70 80 90 100 110 120 GYNGNSELQY VSYRLGETIF DVKECQIRGA TYSAALRVKL RLVIYERDIL EATVKDIKEQ 130 140 150 160 170 180 EVYMGEIPLM TNNGTFIING TERVVVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR 190 200 210 220 230 240 GSWLDFEFDP KDNLFVRIDR RRKLPVSIIL RALNYNTEEI LNIFFEKNIF KIENNKIVLE 250 260 270 280 290 300 LVPERLRGET ASFNIKKNGI IYVEKGRRIT AKHIQELKNN KIKSITVPVE YILGRIVSKN 310 320 330 340 350 360 YVDKKTGETI ISSNTELSLE ILEKLKKLGF YSIETLFTND LDHGPYISET LRIDSSNDRM 370 380 390 400 410 420 SALIEIYRVM RPGEPPTKEA TENLFENLFF SEDRYDLSTV GRMKFNRSLL REETKGSSTL 430 440 450 460 470 480 NKEDIIDVIK KIIDIRNGKG EVDDIDHLGN RRVRSVGEMA ENQFRIGLVR VERAVKERLS 490 500 510 520 530 540 IGDLDALMPQ DMINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGLGGLTR 550 560 570 580 590 600 ERAGFEVRDV HPTHYGRVCP IETPEGPNIG LINSLSVYAQ TNSHGFLETP YRKVCNGLVS 610 620 630 640 650 660 EEIHYLSAIE EGNYIIAQAN TNIDKTGFFT DDLVTCRHKG ESSLFNRNQV NYMDVSTQQI 670 680 690 700 710 720 VSVGASLIPF PEHDDANRAL MGANMQRQAV PTLKTDKPLI GTGMERAVAV DSGVTAVAKR 730 740 750 760 770 780 SGVVQYVDAS RIVIKVDEKE MYSREAGIDI YNLTKYTRSN QNTCINQQPC VNLGEKIKKG 790 800 810 820 830 840 DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELSCIS 850 860 870 880 890 900 RDTKLGAEEI SSDIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK 910 920 930 940 950 960 LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQIFTRDGVK KDKRALEIED MQLKKIKQDL 970 980 990 1000 1010 1020 TEEFKIFESS LFTHIKKTFI SLDIETKQLD TLPFEKWFSV DIKQKDKKKE IEKLAKQHHE 1030 1040 1050 1060 1070 1080 LKEEFNKKIE IKRQKITQGD DLAPGVLKIV KVYLAVKRQI QPGDKMAGRH GNKGVISKIN 1090 1100 1110 1120 1130 1140 PVEDMPYDEN GIPVDIVLNP LGVPSRMNIG QILETHLGMA AKGIGDKINH MLKTQEKISN 1150 1160 1170 1180 1190 1200 LRKFIQKAFD LGDNLRQKVN LDTFSNEEIL RLAKNLKNGI PISTPVFDGA QENEIKQMLK 1210 1220 1230 1240 1250 1260 FAGLPTSGQI ILFDGRTGEK FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG 1270 1280 1290 1300 1310 1320 GKAQFGGQRF GEMEVWALEA YGASYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP 1330 1340 ESFNVLLKEI RSLGINIELE SE

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12761.1.
RefSeq	NP_239875.1.
3D structure databases
HSSP	HSSP built from PDB template 1HQM based on UniProtKB Q9KWU7.
ModBase	Search...
Genome annotation databases
GeneID	1109542.
GenomeReviews	Gene locus BU034 in contig BA000003_GR.
KEGG	buc:BU034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57146.
Enzyme and pathway databases
BioCyc	BSP107806:BU034-MON.
Family and domain databases
HAMAP	MF_01321. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.150. Ribosomal_L2. 1 hit.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. rpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProDom	P57146. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RPOB_BUCAI

Accession

Primary (citable) accession number: P57146

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)