Reviewed, UniProtKB/Swiss-Prot P57153 (MURB_BUCAI)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BU045

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP(+) = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH.
Cofactor	FAD By similarity.
Pathway	Cell wall biosynthesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 356	356	UDP-N-acetylenolpyruvoylglucosamine reductase
Regions
	Domain	20 – 190	171	FAD-binding PCMH-type
Sites
	Active site	166	1	By similarity
	Active site	236	1	Proton donor By similarity
	Active site	331	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57153-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 103486BF1DE25EA7	FASTA	356	40,593
	10 20 30 40 50 60 MHKKKYFSSQ SLKDLNTFAI DVTAKKIIFV KTIQSLIDIS KICQLSNIPY IILGEGSNVL 70 80 90 100 110 120 FLENYVGVVI INRIKGIKIV EKKNFWLLHV FSGEKWHNLV KLTLNLGILG LENLALIPGC 130 140 150 160 170 180 IGSAAIQNIG AYGLEFKDVC QYVDILSLKD NTIKRIYKNS CKFSYRNSIF KDQYKNEHAV 190 200 210 220 230 240 IRVGIKLSKK WRPILFSSLE NYITPINITA YKIFNIICKI RKKKLPDPKK IGNAGSFFKN 250 260 270 280 290 300 PLIKKKKAQK LINLYKVPNY PQKNGLVKIS AAWLIENYKF KHLQIGDAAI HKKQKLILIN 310 320 330 340 350 KKNATAQEII KLAKIIHKCI LKKFNILLEP EVDLIGASGK IKASKIFKLN SKLKVI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12768.1.
RefSeq	NP_239882.1.
3D structure databases
HSSP	HSSP built from PDB template 2MBR based on UniProtKB P08373.
ModBase	Search...
Genome annotation databases
GeneID	1109549.
GenomeReviews	Gene locus BU045 in contig BA000003_GR.
KEGG	buc:BU045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57153.
Enzyme and pathway databases
BioCyc	BSP107806:BU045-MON.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DHase_flavot_FAD-bd_sub2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProDom	P57153. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MURB_BUCAI

Accession

Primary (citable) accession number: P57153

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)