Reviewed, UniProtKB/Swiss-Prot P57157 (ARGB_BUCAI)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name(s)=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	BU049

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 257	257	Acetylglutamate kinase
Regions
	Nucleotide binding	180 – 185	6	ATP By similarity
	Nucleotide binding	208 – 210	3	ATP By similarity
	Region	43 – 44	2	Substrate binding By similarity
Sites
	Binding site	65	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Site	7	1	Transition state stabilizer By similarity
	Site	216	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57157-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 54EF26B46D6C064B	FASTA	257	27,811
	10 20 30 40 50 60 MNPLVIKLGG VLLESDDAMK RLFEALVDYQ KFYKRHVSVI HGGGRLIDNL MNKLSLPVKK 70 80 90 100 110 120 KNGLRITPSE HINIITGALA GTANKTLLAW ALKYNINAIG LCLADGGSVD VERLDENLGH 130 140 150 160 170 180 VGKAIPGSPL FLKKLFKEGT IPIISSIGIT KDGLLMNVNA DLAATALATT LQANLILLSD 190 200 210 220 230 240 ISSILDGKGQ RITEIDSIQA EKLIMQGIIT NGMIVKVRAA LEAARVLRRP VDIASWQNTE 250 KLKLLFNGVN IGTRVYV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12772.1.
RefSeq	NP_239886.1.
3D structure databases
HSSP	HSSP built from PDB template 1GS5 based on UniProtKB P11445.
ModBase	Search...
Genome annotation databases
GeneID	1109456.
GenomeReviews	Gene locus BU049 in contig BA000003_GR.
KEGG	buc:BU049.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57157.
Enzyme and pathway databases
BioCyc	BSP107806:BU049-MON.
Family and domain databases
HAMAP	MF_00082. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProDom	P57157. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

ARGB_BUCAI

Accession

Primary (citable) accession number: P57157

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)