Reviewed, UniProtKB/Swiss-Prot P57166 (GCP_BUCAI)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	BU058

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated.
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Uncategorized	O-sialoglycoprotein endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 336	336	Probable O-sialoglycoprotein endopeptidase
Sites
	Metal binding	110	1	Zinc Potential
	Metal binding	114	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	P57166-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 11FE9A328D885971	FASTA	336	37,438
	10 20 30 40 50 60 MRILGIETSC DDTGIAIYDT NKGLLINEIY NQRKLNNIYG GIIPELASRE HMEAMIVLLN 70 80 90 100 110 120 KIFKKKNIYK YVDMIAYTAG PGLIGSLLVG ATFACSLGLS LNIPVLPVHH MEAHLLSPML 130 140 150 160 170 180 DYKTIQFPFI GLLVSGKHTQ IIGAHKFGEY EILGNCLDDA AGEAFDKTAK LLGLKYPGGL 190 200 210 220 230 240 ELSKLASKGI KDYFYFPRPM IHHSDLNFSF SGLKTFAAQT IKKSSKSMQE KANIAKAFED 250 260 270 280 290 300 AVIDILLIKT KKALKKQKWK RLVIAGGVSA NQKLRKKSEI MVKKNFNGTV FYSSLEFCTD 310 320 330 NAAMIAYLGS LRQKEARNSQ LEILVKPKWS IDDLCF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12781.1.
RefSeq	NP_239895.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	1109465.
GenomeReviews	Gene locus BU058 in contig BA000003_GR.
KEGG	buc:BU058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57166.
Enzyme and pathway databases
BioCyc	BSP107806:BU058-MON.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

GCP_BUCAI

Accession

Primary (citable) accession number: P57166

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)