Reviewed, UniProtKB/Swiss-Prot P57172 (DNLJ_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]

Gene names

Name:	ligA
Synonyms:	lig
Ordered Locus Names:	BU067

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.
Catalytic activity	NAD(+) + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m).
Sequence similarities	Belongs to the NAD-dependent DNA ligase family. LigA subfamily. Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Ligand	NAD
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA ligase (NAD+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 675	675	DNA ligase
Regions
	Domain	595 – 675	81	BRCT
Sites
	Active site	115	1	N6-AMP-lysine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57172-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 3C77E54F6F0ADC24	FASTA	675	77,536
	10 20 30 40 50 60 MTSTKDKIEK LRKILLKYEY FYHTLNQSII SDAEYDYLFR QLYELELKNK ELIPSDSPTQ 70 80 90 100 110 120 KVGSHILQKF KKIKHFSPML SLENTFDVNG YLNFKKRIKK SIHNNEPLSF CCELKLDGVA 130 140 150 160 170 180 ISIIYEEGIF VRAATRGDGF EGENITSNAR MIDSIPLKLK GIDIPKRLEI RGEVFMLKSN 190 200 210 220 230 240 FIKLNKKYKL NQNKYFSNPR NAAAGSLRHI DPNITAERKL IFSCHGCDFF VKTNKELTTH 250 260 270 280 290 300 YQRLMKCLSW GIPVNKEIVI CSSDIEIIQF YKKIAQKRNF LDFDIDGIVI KVNSLELQKK 310 320 330 340 350 360 IGSTTKSPRW AIAFKFSPKE RITTLNDVKF QVGRTGVITP VAYFNPVYIS GVMISKASLH 370 380 390 400 410 420 NKNEIERLNL HFNDTITICR SGDVIPRLLN VIEIRRCDNA KKIIFPSFCP VCNTELLENI 430 440 450 460 470 480 EEKLIRCHSG LTCDAQKKQA LYHFFSKKSL YVVGLGPKII NELVEKGLVK NPIDFFYLKD 490 500 510 520 530 540 IDLIQLKNVG KRKSIKIINS IKKCKKTTLK CFIYALGIPG VGEVVAGKIA NYFIKLDKLM 550 560 570 580 590 600 NSNILELNCI SGVGKIISNN IFNYFSTISN REMVVKLIKQ AGIFLNDQEI HKINSEKTYF 610 620 630 640 650 660 FNKKIVLTGV FKSFSRIELK TILLSLGAKI SNNISRKTDF LIYGNNFGSK FFRAKDLDVK 670 IINQEELNSL IRIKE

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12790.1.
RefSeq	NP_239904.1.
3D structure databases
HSSP	HSSP built from PDB template 1B04 based on UniProtKB O87703.
ModBase	Search...
Genome annotation databases
GeneID	1109474.
GenomeReviews	Gene locus BU067 in contig BA000003_GR.
KEGG	buc:BU067.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57172.
Enzyme and pathway databases
BioCyc	BSP107806:BU067-MON.
Family and domain databases
HAMAP	MF_01588. [Tree]
InterPro	IPR001357. BRCT. IPR004150. DNA_ligase_OB. IPR001679. DNAligase. IPR013839. DNAligase_adenylation. IPR013840. DNAligase_N. IPR003583. HHH1_bd. IPR012340. NA-bd_OB-fold. IPR004149. Znf_DNAligase_C4. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF01653. DNA_ligase_aden. 1 hit. PF03120. DNA_ligase_OB. 1 hit. PF03119. DNA_ligase_ZBD. 1 hit. [Graphical view]
PIRSF	PIRSF001604. LigA. 1 hit.
ProDom	PD003944. DNAligase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00292. BRCT. 1 hit. SM00278. HhH1. 3 hits. SM00532. LIGANc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00575. dnlj. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS01055. DNA_LIGASE_N1. 1 hit. PS01056. DNA_LIGASE_N2. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DNLJ_BUCAI

Accession

Primary (citable) accession number: P57172

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)